Optical waveguide fluorescence enhanced detector

Abstract

Fluorescence is long wavelength light slowly released after short wavelength of light illumination, and fluorescence detection technology belongs to the field of physical measurement. The invention provides an optical waveguide fluorescence enhanced detector, which comprises a hollow metal clad waveguide, a laser, a spectrometer, and a peristaltic pump for sample input and output and flushing. The detector is characterized in that: the free space coupling technique is employed to excite an ultrahigh-order mode in the hollow metal clad waveguide, most of the light can be coupled into the waveguide, so that the optical power density in a sample serving as a waveguide layer can be greatly increased, thus enhancing the fluorescence excitation efficiency. The detector has the advantages that: (1) the attenuated total reflection effect caused by ultrahigh-order mode excitation greatly weakens the intensity of excitation wavelength in a reflection spectrum; (2) because of the enhancement effect of a fluorescence signal, the detector can detect solutions and gas sample with very low concentration; (3) due to wavelength difference, the fluorescence signal can be radiated by a reflection angle different from excitation light; and (4) the structure is simple, the operation is convenient, and the cost is low.

Description

technical field [0001] Fluorescence spectroscopy has the advantages of high sensitivity, good selectivity, and fast detection speed, and can provide a lot of information about the molecular structure and electronic state of the sample. It has become an important trace analysis technique and belongs to the field of physical measurement. Background technique [0002] With the rapid development of my country's national economy and the continuous increase of population, environmental pollution has become a major problem that seriously damages people's health. More and more people are beginning to pay attention to the toxic and harmful gases in the atmosphere, as well as the organic matter and heavy metals in rivers, lakes and oceans. Ionic water pollution. The precise qualitative and quantitative detection of toxic substances in the atmosphere and water has become an important topic. [0003] The interaction of biomolecules and the molecular structure changes caused by chemical ...

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Problems solved by technology

However, there are still the following disadvantages in this type of liquid core waveguide technology: first, the excitation light and fluorescence signals are output from the same end face, and the separation of excitation light and fluorescence cannot be achieved well, which greatly interferes with the detection results; secondly, due to The refractive index of the solution is close to that of water, and the cost of making a solid thin layer material lower than the refractive index of water is relatively high; third, this type of liquid-core optical fiber cannot detect the fluorescence of gas samples

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