Method of breaking dormancy of ryegrass endophytic fungi symbiont seeds

A technology of endophytic fungi and symbionts, applied in seed and rhizome treatment, application, agriculture, etc., can solve the problems of enhancing the dormancy characteristics of host grasses, and achieve the effect of improving germination rate, high safety, and slowing damage.

Active Publication Date: 2017-04-26
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, endophytic fungal infection often enhances the dormancy characteristics of host grasses. At present, there is no mature method to solve this problem to effectively improve the germination rate and emergence uniformity of ryegrass endophytic fungal symbiont cultivation grassland. And other issues

Method used

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  • Method of breaking dormancy of ryegrass endophytic fungi symbiont seeds

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Effect test

Embodiment 1

[0034] Embodiment 1 Comparative example

[0035] The method steps for breaking the seed dormancy of the ryegrass endophytic fungus symbiont are as follows:

[0036] (1) Take 5 g of dormant ryegrass endophyte symbiont seeds and place them in a 200 ml beaker;

[0037] (2) Add 100 ml of 50% concentrated sulfuric acid to the beaker containing the seeds, and stir continuously with a glass rod to completely soak the seeds in the concentrated sulfuric acid;

[0038] (3) After 10 minutes, filter out the concentrated sulfuric acid and rinse the seeds with tap water;

[0039] (4) As a control, the seeds were evenly placed in a petri dish covered with two layers of sterilized filter paper for TP germination, with 50 seeds per dish, 4 replicates, and cultured under dark conditions at 25±1°C. Observe and count the germination situation every day, and after 14 days, the germination ends, and the germination potential and germination rate are counted.

Embodiment 2

[0041] The method steps of breaking the dormancy of ryegrass endophytic fungus symbiont seeds of the present invention are as follows:

[0042] (1) Take 5 g of dormant ryegrass endophyte symbiont seeds and place them in a 200 ml beaker;

[0043] (2) Add 100 ml of 50% concentrated sulfuric acid to the beaker containing the seeds, and stir continuously with a glass rod to completely soak the seeds in the concentrated sulfuric acid;

[0044] (3) After 10 minutes, filter out the concentrated sulfuric acid and rinse the seeds with tap water;

[0045] (4) Put the rinsed seeds into another clean 200ml beaker, add 150ml of distilled water, and place the beaker together with the beaker in a 4°C refrigerator for low-temperature treatment;

[0046] (5) Filter the seeds after 3 days of low temperature treatment, and place the seeds in distilled water at 40°C for warm water initiation;

[0047] (6) Filter the seeds after 10 minutes, place the seeds evenly in a petri dish covered with two...

Embodiment 6

[0055] The method steps of breaking the dormancy of ryegrass endophytic fungus symbiont seeds of the present invention are as follows:

[0056] (1) Take 5 g of dormant ryegrass endophyte symbiont seeds and place them in a 200 ml beaker;

[0057] (2) Add 100 ml of 50% concentrated sulfuric acid to the beaker containing the seeds, and stir continuously with a glass rod to completely soak the seeds in the concentrated sulfuric acid;

[0058] (3) After 9 minutes, filter out the concentrated sulfuric acid and rinse the seeds with tap water;

[0059] (4) Put the rinsed seeds in another clean 200ml beaker, add 150ml of distilled water, and place the beaker together with the beaker in a 3°C refrigerator for low-temperature treatment;

[0060] (5) Filter the seeds after 6 days of low temperature treatment, and place the seeds in distilled water at 39°C for warm water initiation;

[0061] (6) Filter the seeds after 11 minutes, place the seeds evenly in a petri dish covered with two la...

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Abstract

The invention provides a method of breaking dormancy of ryegrass endophytic fungi symbiont seeds. The method includes the steps of: treating dormant ryegrass endophytic fungi symbiont seeds in concentrated sulfuric acid being 50% in volume concentration for a certain time, washing the concentrated sulfuric acid away, adding water until the seeds are all immersed, performing low-temperature treatment for a certain time, and priming the seeds in warm water at 40 +/- 1 DEG C. The method has the following advantages: (1) according to the steps, the method can quickly break dormancy of the seeds within a short time; (2) the method is safe, wherein during the dormancy breaking process of the seeds, the concentration of the sulfuric acid is low and use amount is low, so that the method has high safety during operation; (3) the method is simple in operations and only includes the steps of soaking the seeds in the concentrated sulfuric acid, storing the seeds at low temperature in a wet condition, and priming the seeds; and (4) germination of the seeds is improved since the seeds is primed in warm water after the low temperature treatment, so that germination rate of the seeds is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of plant-microbe interaction and seed physiology, and in particular relates to a method for breaking seed dormancy of ryegrass endophytic fungus symbiont. Background technique [0002] Ryegrass ( Lolium perenne ) is native to Western Europe, North Africa, and Southeast Asia. It was first domesticated as pasture grass in the United Kingdom, and is now widely planted as an excellent lawn and pasture in various temperate regions of the world. Ryegrass has excellent turf properties such as large thousand-grain weight, high germination rate, fast formation, many tillers, strong regeneration ability, mowing resistance, and soft texture. It plays a pivotal role in the establishment of artificial grassland in temperate zones. At the same time, it has extremely high biomass under suitable environmental conditions, high utilization rate of nitrogen, good palatability, strong regeneration ability after grazing, trampl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 李秀璋李春杰魏学凯陈振江
Owner LANZHOU UNIVERSITY
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