The invention belongs to the technical field of plant nutrient liquid and particularly discloses nutrient liquid capable of improving resistance of cucumbers as well as a preparation method and application thereof. The nutrient liquid comprises gamma-aminobutyric acid, potassium nitrate, calcium nitrate tetrahydrate, monopotassium phosphate, magnesium sulfate heptahydrate, trace elements and the like, and comprises macroelements such as N, P, K, Ca, Mg and S. When the nutrient liquid is applied to the fruiting period of the cucumbers, the picking period of the cucumbers can be significantly prolonged, the malformation rate can be reduced, the fruit yield of single plant of cucumber can be increased, and the content of vitamin C, soluble protein and free amino acid of the cucumbers can be significantly increased; in addition, when the nutrient liquid is applied to the fruiting period of the cucumbers, the nitrate content of the cucumbers can be reduced, the net photosynthetic rate of the cucumbers is increased, intercellular carbon dioxide concentration and the stomatal conductance are increased, element deficiency symptoms in the middle and later fruiting period of the cucumbers are avoided, and the high-temperature resistance and the extreme environment resistance of the cucumbers are significantly enhanced.