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Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof

A technology of Bacillus campylobacter and HL-37 is applied in the field of soil pollution biological treatment to achieve the effects of promoting plant growth, improving efficiency and increasing the activity of cadmium

Inactive Publication Date: 2017-04-26
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the research on screening out Bacillus curvedus from farmland soil and specifically using Bacillus curvedus to activate heavy metal cadmium has not been reported.

Method used

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  • Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof
  • Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and Identification of Bacterial Strains

[0026] A high-efficiency cadmium-soluble Campylobacter bacterium provided by the present invention is screened in the following manner. Collect the rhizosphere soil of amaranth polluted by heavy metal cadmium, according to the plate dilution method, weigh 10 grams of fresh soil samples in a 250 Erlenmeyer flask containing 90 mL of sterile water and glass beads, make a bacterial suspension, and dilute to 10 -4 -10 -6 times, spread on insoluble solid medium, and incubate upside down at 28°C for 3-5 days. After a single colony grows on the plate, pick a single colony with an obvious phosphorus-dissolving circle for multiple streaking and purification ( figure 1 ), and obtained a strain of bacteria, numbered HL-37. The bacterial strain was preserved in the Guangdong Provincial Microbiological Culture Collection Center (GDMCC) on June 13, 2016. The preservation number is GDMCC No.60045, and the preservation a...

Embodiment 2

[0029] Embodiment 2: the determination of the ability of activating cadmium carbonate by bacillus flexus HL-37

[0030] The screened Bacillus flexus has a better effect of activating cadmium carbonate through the following experiments. The bacteria HL-37 stored on the slant at 4°C were activated, inoculated on the activation medium, and cultivated for 24 hours. Pick the cadmium-dissolving bacteria on the activation medium and inoculate it in the enrichment medium, culture at 28°C, 150r / min, for 14-18h. Obtain the enrichment solution or make a bacterial agent, and the number of bacterial cells can reach 10 by plate counting 10 CFU / ml. The activation medium is broth peptone medium. The enrichment solution was inoculated into cadmium-dissolving medium (without water-soluble cadmium ions) medium at a volume ratio of 2%, and cultured in a constant temperature incubator at 28°C and 150r / min for 3 days, and a sterile control was set at the same time. After 3 days, the culture was...

Embodiment 3

[0031] Example 3: Determination of the ability of Bacillus flexus HL-37 to activate cadmium phosphate

[0032] The screened Bacillus flexus has a better ability to activate cadmium phosphate through the following experiments. The bacteria HL-37 stored on the slant at 4°C were activated, inoculated on the activation medium, and cultivated for 24 hours. Pick the cadmium-dissolving bacteria on the activation medium and inoculate it in the enrichment medium, culture at 28°C, 150r / min, for 14-18h. Obtain the enrichment solution or make a bacterial agent, and the number of bacterial cells can reach 10 by plate counting 10 CFU / ml. The activation medium is broth peptone medium. The enrichment solution was inoculated in cadmium-dissolving medium (without water-soluble cadmium ions) at a volume ratio of 2%, and cultured in a constant temperature incubator at 28°C and 150r / min for 3 days, and a sterile control was set at the same time. After 3 days, the culture was terminated, and th...

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Abstract

The present invention belongs to the technical field of soil contamination biological treatment, and specifically discloses Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, wherein the Bacillus flexus HL-37 is preserved in Guangdong culture collection center on June 13, 2016, and has the preservation number of GDMCCNo.60045. According to the present invention, the aboriginal microorganism having the efficient cadmium activation function is screened from the farmland soil, and the Bacillus flexus HL-37 can activate the soil mineral nutrients at the rhizosphere, promote the plant growth, and effectively dissolve the carbonate binding state cadmium, the phosphate binding state cadmium and the cadmium having other main binding states in the soil, such that the soil heavy metal cadmium restoration efficiency of the super-accumulation plant is effectively improved; and during the soil heavy metal cadmium activation process with the strain, any discharged environmental pollutants are not produced, and the Bacillus flexus HL-37 has great application prospects in preparation of environment-friendly soil improvers.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of soil pollution, and more specifically relates to a Bacillus flexus that can activate soil heavy metal cadmium ( Bacillus flexus ) HL-37 and its application. Background technique [0002] With the development of modern industry, the problem of soil quality deterioration caused by heavy metals entering the environment is becoming more and more serious. According to the survey by the Ministry of Agriculture, the area of ​​land polluted by heavy metals in my country accounts for 64.8% of the total area of ​​sewage irrigation areas, seriously threatening agricultural production and human health. [0003] Phytoremediation of heavy metal contaminated soil has gradually attracted people's attention due to its advantages of economy, environmental friendliness, and large amount of remediation. However, there are still many limitations in using plants, especially hyperaccumulators, to contr...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10C12N1/20C12N1/205C12R2001/07
Inventor 王立立李取生周雪芳何宝燕周婷
Owner JINAN UNIVERSITY
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