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Method for breeding lasting disease-resistant hybrid rice mixing line

A hybrid rice, long-lasting technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., to achieve the effect of easy resistance gene combination, reduced selection pressure, and neat agronomic traits

Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports at home and abroad on the use of maintainer lines containing multiple resistance genes to breed disease-resistant sterile lines and to prepare hybrid rice disease-resistant mixed lines to control rice diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (Note that these resistant varieties can be found in the National Rice Data Center, and Yixiang 1B is the maintainer line of Yixiang 1A)

[0027] 1) Select the rice blast resistance variety LAC23 (containing the rice blast resistance gene Pi-1 ), rice blast resistant variety 75-1-127 (containing the rice blast resistance gene Pi-9 ) and rice blast resistance variety Sanhuangzhan 2 (containing blast resistance gene Pi-56 ) were respectively crossed with the maintainer line Yixiang 1B (http: / / www.ricedata.cn / variety / varis / 609023.htm, which can be found in the National Rice Data Center), and the hybrid F 1 Substituted by F 1A (genotype is Pi-1pi-1pi-9pi-9pi-56pi-56 ), F 1B (genotype is pi-1 pi-1Pi-9pi-9pi-56pi-56 ) and F 1C (genotype is pi-1pi-1pi-9pi-9Pi-56pi-56 )express;

[0028] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1A / F 1B , F 1A / F 1C Have to repay PYF 1 , to PYF 1AB (genotype is Pi- 1pi-1Pi-9pi-9pi-56pi-56 ) and PYF ...

Embodiment 2

[0039] 1) Select a bacterial blight-resistant variety Huangyu (containing the bacterial blight-resistant gene Xa-1 ), bacterial blight variety CBB23 (containing bacterial blight resistance gene Xa-23 ) and the bacterial blight-resistant variety IRBB27 (containing the bacterial blight-resistant gene Xa-27 ) was crossed with the fine maintainer line Yixiang 1B to obtain the hybrid F 1 Substituted by F 1A (genotype is Xa-1xa-1xa-23xa-23xa-27xa-27 ), F 1B (genotype is xa- 1xa-1Xa-23xa-23xa-27xa-27 ) and F 1C (genotype is xa-1xa-1xa-23xa-23Xa-27xa-27 )express;

[0040] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1A / F 1B , F 1A / F 1C Have to repay PYF 1 , to PYF 1AB (genotype is Xa- 1xa-1Xa-23xa-23xa-27xa-27 ) and PYF 1AC (genotype is Xa-1xa-1xa-23xa-23Xa-27xa-27 )express;

[0041] 3) Planting PYF 1AB and PYF 1AC , at the seedling stage, the molecular markers linked to the disease resistance gene were screened in two recrossed pop...

Embodiment 3

[0051] 1) Select the rice blast resistance variety LAC23 (containing the rice blast resistance gene Pi-1 ), rice blast resistant variety 75-1-127 (containing the rice blast resistance gene Pi-9 ), rice blast resistance variety Sanhuangzhan No. 2 (contains rice blast resistance gene Pi-56 ) and rice blast resistant variety BL1 (containing blast resistance gene Pi-b ) were respectively crossed with the excellent maintainer line Yixiang 1B to obtain the hybrid F 1 Substituted by F 1A (genotype is Pi-1 pi-1 pi-9 pi-9 pi-56pi-56 pi-b pi-b ), F 1B (genotype is pi-1 pi-1Pi-9 pi-9 pi-56 pi-56 pi- b pi-b ), F 1C (genotype is pi-1pi-1pi-9pi-9Pi-56pi-56 pi-b pi-b ) and F 1D (genotype is pi-1pi- 1pi-9pi-9pi-56pi-56Pi-b pi-b )express;

[0052] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1A / F 1B , F 1C / F 1D Have to repay PYF 1 , to PYF 1AB (genotype is Pi-1 pi-1 Pi-9 pi-9 pi-56 pi-56 pi-b pi-b ) and PYF 1CD (genotype is pi-1 pi-1 pi-9 ...

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PUM

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Abstract

The invention discloses a method for breeding a lasting disease-resistant hybrid rice mixing line and belongs to the field of hybrid rice disease resistance breeding. A plurality of resistance genes are led into an excellent maintenance line through a molecular marker auxiliary back cross breeding method, and a disease-resistant sterile line containing heterozygous disease-resistant genes is obtained through the breeding of the maintenance line and an original sterile line. Hybrids containing equal-proportion multiple disease-resistant gene combinations can be obtained by production of hybrid seeds from the disease-resistant sterile line and a restoring line. The hybrids are composed of single plants containing multiple resistance genes, single plants containing single resistance genes and disease-resistant single plants. The hybrids are mixed populations of various resistance level plants, selection pressure on pathogenic bacteria caused by vertical resistance can be avoided or reduced, and the goal of lasting disease resistance is achieved.

Description

technical field [0001] The invention specifically relates to a method for breeding hybrid hybrid rice lines with persistent disease resistance, and belongs to the technical field of disease resistance breeding. Background technique [0002] The main measure to prevent the loss of disease resistance of rice varieties and improve the resistance persistence of rice varieties should be to prevent pathogenic bacteria from producing new pathogenic races or prevent the emergence of new dominant races as much as possible. Under the current technical conditions, there are two strategies to prevent pathogenic bacteria from producing new pathogenic races or new dominant races, one is resistance gene aggregation, and the other is resistance gene mixing. [0003] Resistance gene aggregation is the aggregation of resistance genes scattered in different varieties into the same genome. The resistance spectrum and resistance are improved after the aggregation of multiple disease resistance ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 官华忠毛大梅陈志伟潘润森吴为人
Owner FUJIAN AGRI & FORESTRY UNIV
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