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Method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock

A technology of star-spotted flounder and reduced gynonuclei, which is applied in the field of breeding of new germplasm of star-spotted flounder, can solve problems that have not been reported, and achieve the effects of improving breeding efficiency, reducing deformity rate, and increasing growth speed

Active Publication Date: 2017-05-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

Luckenbach et al. (2004) from North Carolina State University used homologous and heterologous sperm to induce gynogenesis in desert flounder (Paralichthys lethostigma), and Piferrer et al. studied turbot gynogenesis (Institut de Ciències del Mar, Consejo Superior de Investigaciones Cientificas, Piferrer et al., 2004), but the method of gynogenesis of star spotted plaice has not been reported at home and abroad

Method used

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  • Method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock

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Embodiment 1

[0036] 1. Establishment of perch sperm freezer

[0037] In 2010, more than 50 sexually mature male perch were collected from farms along the coast of the Bohai Sea, and they were bred in the factory flow pond of Haiyang Huanghai Fisheries Co., Ltd. Every autumn, when the water temperature drops below 18°C, the male gonads mature. You can select the male perch that has developed to produce semen, squeeze its abdomen to collect semen, use a microscope to detect that the sperm motility reaches more than 80%, and carry out cryopreservation; Mixed at a ratio of 1:1, divided into 2.0mL cryopreservation tubes, and then placed in liquid nitrogen (-196°C) for cryopreservation in large quantities. In 2014 and 2015, more than 400 copies of sperm were cryopreserved, and sea bass sperm freezing was established. The library achieves the purpose of long-term mass preservation and application.

[0038] The concentration of each component of sperm diluent LJS is:

[0039] NaCl 3.526g / L, NaH ...

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Abstract

The invention discloses a method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock, comprising the steps: (1) establishing sperm cold storage for perches, (2) inactivating sperm genetic substances, (3) collecting and impregnating unfertilized eggs of Platichthys stellatus Pallas, (4) conducting induction of pressure shock towards fertilized eggs, and (5) cultivating gynogenetic eggs.The method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock has the advantages of improving the fertility rate and developmental rate of Platichthys stellatus Pallas's gynogenesis and establishing gynogenetic groups of Platichthys stellatus Pallas.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a breeding technique for cultivating all-female, fast-growing new germplasm of star-spotted flounder. Background technique [0002] Star spotted flounder (Platichthys stellatus) is mainly distributed along the coasts of Asia and America in the North Pacific, and the main distribution countries are Canada, the United States, Russia, China, North Korea, South Korea and Japan. Star spotted flounder has strong tolerance to changes in water temperature and salinity, adapts to a wide range of temperatures, has strong resistance to stress, has a high survival rate in breeding, has a thick body, bright and pleasing body color, and has extremely high economic value. Turbot (Scophthalmus maximus), flounder (Paralichthysolivaceus) and half-smooth tongue sole (Cynoglossus semilaevis) are excellent species with great breeding potential, and they are also one of the important...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 田永胜李文龙段会敏李振通唐江成美玲陈张帆
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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