Colorectal cancer liver metastasis animal model construction method for multi-modal imaging research

Abstract

The invention discloses a colorectal cancer liver metastasis animal model construction method for multi-modal imaging research. The defect that an existing colorectal cancer liver metastasis implanting model is high in colorectal obstruction morbidity and short in model survival time and cannot adapt to long-term experiment is overcome. Adopted animals are 5-year-old BALB / C-nu-nu female nude mice, an adopted injection is human colorectal cancer cells (HT29), and the method comprises the construction steps that 0.1 ml of colorectal cancer cells with the concentration being 2.5*10<7> / ml prepared from 0.01 MPBS is injected into the spleen, the injection time is about 3 min, the spleen is placed into an enterocoelia after injection is finished, and a colorectal cancer liver metastasis nude mouse model can be obtained seven weeks after the model is manufactured. Due to the fact that the concentration of the colorectal cancer cells (HT29) is 25*10<7> / ml, the dosage of the colorectal cancer cells is 0.1 ml, the liver metastasis model can be obtained by only injecting a cell suspension into the spleen at a time, and therefore the method is easy to implement, high in molding success rate and worthy of popularization and has certain economic and practical value.

Description

technical field [0001] The invention relates to the field of liver metastases construction, in particular to a method for constructing an animal model of colorectal cancer liver metastases for multimodal imaging research. Background technique [0002] At present, animal models for liver metastasis of colorectal cancer mainly include implanted colon cancer liver metastasis model, transgenic animal tumor model and induced tumor model, and implanted colon cancer liver metastasis animal models are divided into orthotopic implantation and ectopic Implantation colon cancer liver metastasis model, ectopic implantation model is divided into portal vein ectopic implantation method, liver ectopic implantation method, and tail vein ectopic implantation method. [0003] The orthotopic implantation model refers to the inoculation of tumor cell suspensions or tumor tissue pieces into the cecum or colon. development process. However, the limitation is that the growth and metastasis of th...

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Problems solved by technology

The disadvantages of this model are: (1) the whole process of tumor metastasis could not be simulated, and the initial steps of primary tumor invasion into surrounding tissue and penetration into blood vessels were reduced; (2) the survival time of experimental animals was relatively short; (3) it could not be injected with If there are too many tumor cells, it is easy to cause hepatic vascular embolism and cause nude mice to die

However, the induction period of this method is longer, and there is a certain gap in the rate of liver metastases compared with the transplantation method.

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