Application of verbascoside in preparation of medicine for treating oral squamous cell carcinoma
A technology of verbasin and oral squamous cell carcinoma, applied in the field of medicine
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Embodiment 1
[0030] Example 1 The effect of verbascoside on the proliferation and apoptosis of HN4 cells.
[0031] Experimental purpose: To observe the effect of verbascoside on the proliferation and apoptosis of HN4 cells.
[0032] Experimental cells: HN4 oral squamous cell carcinoma cells, purchased from the Institute of Cells, Shanghai Academy of Biological Sciences.
[0033] Experimental drugs: verbascoside was purchased from the Northwest Institute of Plateau Biology, Chinese Academy of Sciences, dissolved in sterile PBS (phosphate buffer saline), and the negative control was PBS.
[0034] Experimental method: Using MTT colorimetry and AO / EB staining techniques, it was shown that verbascoside can effectively inhibit the proliferation of HN4 cells and induce apoptosis of HN4 cells. Specific steps are as follows:
[0035] 1. MTT colorimetric method to detect cell proliferation
[0036] 1) Cell inoculation: HN4 cells were prepared into a single cell suspension with high-sugar DMEM / F-1...
Embodiment 2
[0064] Example 2 Effect of verbascoside on tumor growth in tumor-bearing nude mice.
[0065] The nude mouse model with subcutaneous tumor implantation was used to detect the effect of verbascoside on the growth of HN4 squamous cell carcinoma tumors, and the specific steps were as follows:
[0066] 1. Animal experiments
[0067] Healthy 3-4 week old female BALB / c nude mice were purchased from the Model Animal Institute of Nanjing University, and those with no adverse reactions and normal diet, drinking water and activities were included in the experiment after feeding for 3 days. The collected HN4 cells were resuspended in serum-free high-glucose DMEM / F-12 and counted (approximately 1×10 7 pieces / ml). Take 100μl (about 10 6 cells) were injected subcutaneously in the anterior right armpit of mice, and after 7-10 days, the tumors were uniform in size (volume about 300 mm) 3 ) tumor-planted mice were randomly divided into 2 groups, 10 in each group. Physiological saline (PBS)...
Embodiment 3
[0095] Example 3 The effect of verbascoside on the main organs of tumor-bearing nude mice.
[0096] Using the nude mouse model of subcutaneous tumor implantation in Example 2 to detect the effect of verbascoside on the main organs of tumor-bearing nude mice, the specific steps are:
[0097] 1. Drug administration experiment
[0098] Prepare tumor-bearing nude mice according to the method of the animal experiment in Example 2, and the tumor size is uniform (volume is about 300mm) 3 ) tumor-planted mice were randomly divided into 2 groups, 10 in each group. Physiological saline (PBS) and verbascoside were injected intraperitoneally into the tumor-bearing mice every day for 2 weeks. During this period, the survival of the tumor-bearing mice was monitored. If there was death, it should be dissected immediately to find the cause of death. After 2 weeks of administration, the mice were sacrificed, and the heart, liver and kidney tissues of the mice in each group were taken out and...
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