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Application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective

A technology of peach and polysaccharide, applied in the field of traditional Chinese medicine, can solve problems such as unreported

Inactive Publication Date: 2017-05-10
GUILIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, there are more than 300 articles related to Shixiantao that have been published, but there are no reports on the preparation of liver-protecting drugs and their preparation methods from the extract of Chinese medicine Shixiantao

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  • Application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective
  • Application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective
  • Application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective

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Embodiment Construction

[0015] The content of the present invention will be further described below in conjunction with the examples, but the present invention is not limited thereto.

[0016] Pharmacodynamic experiment methods and results of Shixiantao polysaccharides in the preparation of liver-protecting drugs:

[0017] 1 Materials and methods

[0018] 1.1 Preparation of the test drug Pholodota chinensisLindl. is a plant of the genus Pholodota chinensisLindl. in the family Orchidaceae. Weigh 1,500 g of dried Shixiantao, add 15,000 ml of water, extract with boiling water twice, 1.5 h each time, combine the extracts, concentrate to 220 ml, and use (Sevag method) chloroform-n-butanol (4:1) for multiple extractions , to obtain the protein-removed supernatant, add 95% ethanol to make the alcohol content reach 80%, and place it in a refrigerator at 4 °C for alcohol precipitation for 12 h. Suction filtration, and the filter residue was washed successively with ether, acetone, and absolute ethanol to ob...

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Abstract

The invention discloses application of pholidota chinensis lindl. polysaccharides to preparation of hepatoprotective. Pseudobulbs or whole herb of collected fresh pholidota chinensis lindl. is dried in shade and crushed to obtain coarse pholidota chinensis lindl. powder, water is added according to a material-liquid ratio of 1:10, extraction is performed twice with 1.5h each time by virtue of boiling water, extracting solutions are mixed for concentration, extraction is performed many times with chloroform and n-butanol in a proportion of 4:1 by adopting a Sevag method to obtain supernatant in which proteins are removed, 95 percent ethanol is added to make the ethanol content reach 80 percent, ethanol precipitation is performed on the mixed solution in a 4 DEG C refrigerator for 12h, suction filtration is performed, and filter residues are washed sequentially with ethyl ether, acetone and anhydrous ethanol to obtain crude pholidota chinensis lindl. polysaccharides; the crude pholidota chinensis lindl. polysaccharides are dissolved in water, the obtained solution is loaded into a DE52 type resin chromatographic column subjected to pretreatment with a phosphate buffer solution with pH of 7.5, stands overnight, and is eluted with distilled water at the flow velocity of 1.2ml / min, and eluent is combined and freeze-dried to obtain the pholidota chinensis lindl. polysaccharides. The liver protecting effects of the pholidota chinensis lindl. polysaccharides are developed, and a research foundation is laid for further deep development of the pholidota chinensis lindl. polysaccharides.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, in particular to the preparation of shixiantao polysaccharide and its application in the preparation of liver-protecting medicines. Background technique [0002] The liver is the largest solid organ in the abdominal cavity and is responsible for important physiological functions of the human body. At present, liver disease has become one of the common diseases threatening human health. Liver injury is a common pathological state of liver diseases, and its treatment directly affects the prognosis of liver diseases. In recent years, the treatment of liver injury has attracted much attention, especially the research on traditional Chinese medicine treatment has made great progress. Many traditional Chinese medicines have obvious liver-protecting effects. Whether it is the chemical components of traditional Chinese medicine or the compound formula of traditional Chinese medicine, they ca...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
CPCA61K31/715A61K36/898C08B37/00C08B37/0003
Inventor 段小群张可锋钟明利高雅曹后康黄思茂
Owner GUILIN MEDICAL UNIVERSITY
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