Rabbit trichophyton mentagrophyte keratin culture medium and making method thereof
A kind of technology of Trichophyton mentagrophytes and keratin, applied in Trichophyton mentagrophytes keratin culture medium and production field thereof, can solve the problems of violation of animal welfare, high technical difficulty and high cost, and achieves low cost and simple acquisition. Easy-to-obtain and easy-to-make effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] The production method of keratin protein is: wash the trotter box to remove sundries, meat hooves and residual skin tissue, then remove the horny bottom and horny ball, keep the horny wall pure horny, then cut into pieces, dry, crush, Pass through a 200-mesh sieve at last, dry and store for future use.
[0019] The ingredients contained in the matrix include: 0.3 parts by weight of magnesium sulfate, 0.05 parts by weight of potassium dihydrogen phosphate, 0.005 parts by weight of ferrous sulfate, 0.003 parts by weight of zinc sulfate, 3 parts by weight of sodium dihydrogen phosphate, disodium hydrogen phosphate 2.5 parts by weight, 1000 parts by weight of distilled water.
[0020] The production method of the keratin protein liquid medium is to put 1 g of keratin protein and 1000 mL of matrix into a conical flask, heat to dissolve, then autoclave at 121° C. for 20 minutes, and cool to obtain the keratin protein liquid medium, which is stored at 4° C.
Embodiment 2
[0022] The production method of keratin protein is: wash the trotter box to remove sundries, meat hooves and residual skin tissue, then remove the horny bottom and horny ball, keep the horny wall pure horny, then cut into pieces, dry, crush, Pass through a 200-mesh sieve at last, dry and store for future use.
[0023] The ingredients contained in the matrix include: 0.5 parts by weight of magnesium sulfate, 0.1 parts by weight of potassium dihydrogen phosphate, 0.01 parts by weight of ferrous sulfate, 0.005 parts by weight of zinc sulfate, 3.86 parts by weight of sodium dihydrogen phosphate, disodium hydrogen phosphate 3.97 parts by weight, 1000 parts by weight of distilled water.
[0024] The production method of the keratin protein liquid medium is to put 1 g of keratin protein and 1000 mL of matrix into a conical flask, heat to dissolve, then autoclave at 121° C. for 20 minutes, and cool to obtain the keratin protein liquid medium, which is stored at 4° C.
Embodiment 3
[0026] The production method of keratin protein is: wash the trotter box to remove sundries, meat hooves and residual skin tissue, then remove the horny bottom and horny ball, keep the horny wall pure horny, then cut into pieces, dry, crush, Pass through a 200-mesh sieve at last, dry and store for future use.
[0027] The ingredients contained in the matrix include: 0.5 parts by weight of magnesium sulfate, 0.1 parts by weight of potassium dihydrogen phosphate, 0.01 parts by weight of ferrous sulfate, 0.005 parts by weight of zinc sulfate, 3.86 parts by weight of sodium dihydrogen phosphate, disodium hydrogen phosphate 3.97 parts by weight, 1000 parts by weight of distilled water.
[0028] The production method of keratin agar medium is to heat and dissolve 1g of keratin, 100mL of matrix, and 15g of bacterial culture agar, and then autoclave at 121°C for 20 minutes to obtain a mixed solution; for the culture medium in inclined test tubes, pour the mixed solution into the test ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More