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Kit for rapidly detecting chlamydia trachomatis and gonorrhea and application of kit

A technology of Chlamydia trachomatis and a kit, which is applied in the field of medical testing, can solve the problems of difficult patients, poor precision and accuracy, and long time consumption.

Inactive Publication Date: 2017-05-10
GUANGZHHOU HUAHONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the detection methods of Chlamydia trachomatis and gonorrhea take a long time, and due to the privacy concerns of STD patients, it is difficult to obtain sufficient cooperation from patients
And colloidal gold immunochromatography has been widely used, and its detection method is the most convenient and fast; but colloidal gold immunochromatography can only obtain qualitative detection results, and often has poor precision and accuracy when used for quantitative detection, and Poor stability

Method used

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  • Kit for rapidly detecting chlamydia trachomatis and gonorrhea and application of kit
  • Kit for rapidly detecting chlamydia trachomatis and gonorrhea and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 rapid detection kit

[0027] Preparation of nitrocellulose membrane coated with detection line and quality control line:

[0028] Gonorrhea antigen monoclonal antibody (mouse anti-human monoclonal antibody, purchased from Ai Meijie (abnova) Technology Co., Ltd.), Chlamydia trachomatis antigen monoclonal antibody (mouse anti-human MOMP monoclonal antibody, purchased from Abnova Technology Co., Ltd.) and goat anti-mouse IgG antibody were prepared into solutions, specifically: 2 mg / ml of gonorrhea antigen monoclonal antibody and 1.5 mg / ml of Chlamydia trachomatis antigen monoclonal antibody were diluted with coating 5mg / ml goat anti-mouse IgG antibody was made into a quality control line spray solution with the coating diluent; the membrane liquid volume was 2μl / cm, and they were used as the gonorrhea test line and Chlamydia trachomatis respectively. The test line and the quality control line were sprayed on the nitrocellulose membrane in ...

Embodiment 2

[0041] Embodiment 2 kit linear range, precision and sensitivity test (Neisseria gonorrhoeae)

[0042] Neisseria gonorrhoeae standard strains (NC29400 and NC29403, purchased from China National Institute for the Control of Pharmaceutical and Biological Products) were amplified and purified in the culture medium, and prepared into 0.1, 0.5, 1, 5, 10, 20 and 50 mg / L with PBS buffer The standard solution, standby;

[0043] Test paper card for measurement:

[0044] Detection method: equilibrate the detection reagent and sample to room temperature, take out the test paper card (prepared in Example 1), and lay it flat; accurately draw 100 μl of standard sample, add it to the sample hole of the test paper card, and analyze it with fluorescent immunochromatography after 20 minutes instrument for detection.

[0045] Linearity: establish a standard curve based on the fluorescence intensity value and the corresponding standard concentration. The standard curve of NC29400 is Y (fluores...

Embodiment 3

[0049] Embodiment 3 kit linear range, precision and sensitivity test (chlamydia trachomatis)

[0050] Prepare the Chlamydia trachomatis membrane protein into standard solution of 0.01, 0.05, 0.1, 1, 5, 10 and 20 ng / ml with PBS buffer, and set aside;

[0051] Test paper card for measurement:

[0052] Detection method: equilibrate the detection reagent and sample to room temperature, take out the test paper card (prepared in Example 1), and lay it flat; accurately draw 100 μl of standard sample, add it to the sample hole of the test paper card, and analyze it with fluorescent immunochromatography after 20 minutes instrument for detection.

[0053] Linearity: establish a standard curve with the fluorescence intensity value and the corresponding standard substance concentration, specifically Y (fluorescence intensity)=101.7X+3.8, R2 value>0.999, illustrate that the test paper card of the present invention has good linearity in the range of 0.01-20ng / ml.

[0054] Sensitivity: P...

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Abstract

The invention relates to a kit for rapidly detecting chlamydia trachomatis and gonorrhea and an application of the kit and belongs to the field of medical detection. Specifically, the kit is a test paper card formed by assembling a processed sample pad, a binding pad, a nitrocellulose membrane and a water absorption pad which are in sequential overlap joint on a PVC (printed circuit board), wherein the a rare earth fluorescent microsphere labeled antibody is adsorbed on the binding pad and the nitrocellulose membrane is coated with a detection line and a quality control line.

Description

technical field [0001] The invention relates to a kit for rapidly detecting chlamydia trachomatis and gonorrhea and an application thereof, belonging to the field of medical detection. Background technique [0002] Chlamydia trachomatis (Ct) has a unique developmental cycle and has two forms: Elementary Body and Reticulate Body. Its unique life cycle is a cyclic process in which cells infected by protoplasma develop into reticulum, reticulum divides into protoplasma, and then reinfects cells. Ct is a pathogen that survives in the human body for a long time and spreads widely. It is one of the main pathogens of sexually transmitted diseases (STDs) in my country. It can cause ectopic pregnancy, premature delivery, miscarriage and urinary tract infection under certain conditions. and many other diseases. Studies have found that Ct infection is still related to the onset of many autoimmune diseases, such as chlamydia infection can cause reactive arthritis, Reiter syndrome and s...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/571G01N33/533G01N33/543
CPCG01N33/56927G01N33/533G01N33/54313G01N33/571
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner GUANGZHHOU HUAHONG BIOLOGICAL TECH
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