Artificially synthesized insecticidal gene with high toxicity on lepidoptera pests and application

Abstract

The invention relates to an artificially synthesized insecticidal gene with high toxicity on lepidoptera pests and application, and belongs to the technical field of biological control. The artificially synthesized insecticidal gene has the advantages that a mcry2Ah gene, a mcry2Ah-vp gene and a mcry2Ah-sp gene with high toxicity on the lepidoptera pests are subject to artificial optimizing and modification, and are synthesized into a new DNA (deoxyribonucleic acid) sequence (shown in SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4), a codon subsequence preferable by plants is utilized, and the expression quantity and stable expression characteristic of the mcry2Ah gene, mcry2Ah-vp gene and mcry2Ah-sp gene in transgenic plants are improved; compared with other modified genes, the mcry2Ah-sp gene has higher pest resistance on the resistance lepidoptera pests, and the gene sequence can be used for transforming the plant to prevent and control pests and delay the forming of pest resistance.

Description

technical field [0001] The invention belongs to the technical field of biological control, in particular to an artificially synthesized insecticidal gene sequence with high toxicity to lepidopteran pests and its application. Background technique [0002] Insect pests are an important factor in the reduction of crop production in the world. On average, about 10% of the total grain output is lost every year, and the direct economic loss reaches more than 10 billion US dollars. In the past few decades, the control has mainly relied on chemical pesticides. While making great contributions to agricultural production, chemical pesticides have caused serious consequences such as environmental pollution, human and animal poisoning, and ecological imbalance. In the face of these huge costs, the world is looking for and developing new pest control strategies and technologies. [0003] Bacillus thuringiensis (Bt) is a widely distributed Gram-positive bacteria. While forming spores, i...

AI Technical Summary

Problems solved by technology

However, in transgenic research, it has been found that the insecticidal protein gene derived from Bt is directly transferred into plants, and there are defects such as unstable expression products and low expression levels (van Aarssen et al, 1995, Plant Molecular Biology, 28:513-524 )

Specific problems include: 1) the natural Bt gene contains high AT, more than 60%, and the mRNA expressed by such a gene in the plant is very easy to be degraded by the plant; 2) there are intron cut points similar to eukaryotic genes in the natural Bt gene, Transcription terminator sequence, resulting in incomplete transcription, abnormal splicing of mRNA, etc.; 3) The codons used in natural Bt genes are quite different from those in plants, which will reduce the efficiency of protein translation; 4) Natural Bt genes are used as prokaryotic sources The structure of the gene is significantly different from that of plants and other eukaryotes, such as eukaryotes containing 5'-UTR sequence and polyA tail sequence at the 3' end

There is no report on the transfer of cry2Ah into plants

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