Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Union application of ferroprotoporphyrin and beta-glucuronidase in prostatic cell heterogeneity hyperplasia detection and kit

A detection kit and aldolidase technology, which is applied in the direction of analyzing materials through chemical reactions and observing the impact on chemical indicators, etc., can solve the problems of non-invasive, undiscovered, and sensitivity of detection results. The specificity is not high, to achieve the effect of improving accuracy

Inactive Publication Date: 2017-05-24
山东瓦博格生物技术有限公司
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no convenient, fast, non-invasive, economical and accurate screening method has been found clinically
In addition, the current clinical screening methods for detecting heterogeneous proliferation of prostate cells are all based on the detection of a single marker, the sensitivity and specificity of the detection results are not high, and the diagnosis of prostate cancer is not accurate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Union application of ferroprotoporphyrin and beta-glucuronidase in prostatic cell heterogeneity hyperplasia detection and kit
  • Union application of ferroprotoporphyrin and beta-glucuronidase in prostatic cell heterogeneity hyperplasia detection and kit
  • Union application of ferroprotoporphyrin and beta-glucuronidase in prostatic cell heterogeneity hyperplasia detection and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This embodiment provides a prostate cell heterogeneous hyperplasia detection kit, which uses proheme and β-glucuronidase as markers for combined detection. The detection results of proheme and β-glucuronidase can be used to judge the heterogeneous proliferation of prostate cells such as prostate acinar epithelial cells in the sample.

[0065] The prostate cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme and a β-glucuronidase detection for detecting β-glucuronidase Reagent membrane.

[0066] Wherein, the original hemoglobin detection reagent film provided by the present invention is made according to the following method:

[0067] 1.1. Make phosphate buffer. The buffer is 1.75mol / L, pH 4.5 phosphate buffer.

[0068] 1.2. Add quantitative polyvinylpyrrolidone K30 to the above-mentioned phosphate buffer solution to obtain the first mixed solution, and stir until completely dissol...

Embodiment 2

[0098] This embodiment provides a prostate cell heterogeneous hyperplasia detection kit, which uses proheme and β-glucuronidase as markers for combined detection. Through the detection results of proheme and β-glucuronidase, it is used to judge the heterogeneous proliferation of cells in the sample.

[0099] The prostate cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme and a β-glucuronidase detection for detecting β-glucuronidase Reagent membrane and sample pretreatment solution for sample processing.

[0100] Wherein, the preparation method of the original heme detection reagent membrane, the β-glucuronidase detection reagent membrane and the sample pretreatment solution can refer to Example 1.

[0101] The effect of the prostatic cell heterogeneity detection kit provided in this example is the same as that in Example 1.

Embodiment 3

[0103] This example provides the verification of the sensitivity, specificity and Youden index of proheme and β-glucuronidase as combined markers for detecting heterogeneous proliferation of prostate cells.

[0104] Sensitivity: also known as sensitivity, refers to the proportion of people who are actually sick can be correctly identified as patients by the screening method; specificity: refers to the proportion of people who are actually disease-free by the screening method. The proportion judged as non-patients.

[0105] 3.1 Sensitivity Verification

[0106] 3.1.1 Specimen source: 100 prostatic fluid specimens of prostate cancer patients collected in the clinical laboratory of a medical unit. Prostate cancer was confirmed pathologically.

[0107] 3.1.2 Detection method: Use the prostatic cell heterogeneity detection kit provided in Example 2 to perform combined detection of proheme and β-glucuronidase on the 100 samples above, and perform the combined detection according t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses union application of ferroprotoporphyrin and beta-glucuronidase in prostatic cell heterogeneity hyperplasia detection and a kit, and belongs to the technical field of fluid pathological biopsy detection. Ferroprotoporphyrin and beta-glucuronidase which are used as a union marker are firstly applied to preparation of a prostatic cell heterogeneity hyperplasia detection kit, and the application shows that amazingly, compared with the conventional application that the ferroprotoporphyrin or the beta-glucuronidase is independently used as a marker for detection, the application disclosed by the invention has the advantage that as the ferroprotoporphyrin and the beta-glucuronidase are used as a union marker for detection, the sensitivity, the specificity and the Youden index are obviously improved; therefore, union detection through the ferroprotoporphyrin and the beta-glucuronidase marker is favorable for improving the accuracy of a diagnosis result of prostatic cancer caused by the prostatic cell heterogeneity hyperplasia.

Description

technical field [0001] The invention relates to the technical field of liquid biopsy pathological detection, in particular to the application and kit of the combination of proheme and β-glucuronidase in the detection of heterogeneous hyperplasia of prostate cells. Background technique [0002] The result of prostate cell heteroplasia is prostate cancer. [0003] Prostate cancer is described as a "silent killer". It is not easy to be detected in the early stage. Usually, the disease has developed to an advanced stage when it is diagnosed. 70% of patients are delayed in treatment. Many world celebrities have died of prostate cancer. The main symptoms are hematuria, dysuria and bone pain. Statistics show that prostate cancer has become the second most common cancer in men worldwide, with 1.1 million newly diagnosed prostate cancer patients in 2012, accounting for about 15% of the total number of new cancer cases. In the past ten years, the incidence of prostate cancer in Chin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 温鹏
Owner 山东瓦博格生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com