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Preparation method of PEG (polyethylene glycol) modified proanthocyanidin liposomes

A technology of proanthocyanidins and anthocyanin lipids, which is applied in the field of preparation of PEG-modified proanthocyanidin liposomes, can solve the problems of lipid membrane drug leakage and poor compounding ability of surfactants, and achieve improved storage stability, excellent moisturizing performance, The effect of improving storage stability and sustained-release performance in vitro

Inactive Publication Date: 2017-05-31
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The stability of liposomes is the biggest reason that limits the industrialization of liposomes, including agglomeration caused by uneven particle size distribution during the preparation process, drug leakage caused by lipid membrane instability, poor compounding ability with other surfactants, etc.

Method used

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  • Preparation method of PEG (polyethylene glycol) modified proanthocyanidin liposomes
  • Preparation method of PEG (polyethylene glycol) modified proanthocyanidin liposomes
  • Preparation method of PEG (polyethylene glycol) modified proanthocyanidin liposomes

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preparation example Construction

[0026] A preparation method of PEG-modified proanthocyanidin liposomes, comprising the steps of:

[0027] S1: Mix 0.0048-0.0359 g of proanthocyanidins and 2-20 ml of PEG phosphate buffer to obtain a mixed solution;

[0028] S2: After mixing 0.0096-0.1678g of hydrogenated lecithin and 0.0352-0.1289g of cholesterol, add 10-100ml of anhydrous ethanol and anhydrous ether mixture, and ultrasonically shake at 45-55°C until completely dissolved to obtain a mixture;

[0029] S3: Add the mixed solution obtained in S1 to the mixed solution in S2 at 40-50°C and sonicate for a period of time to form a one-way system;

[0030] S4: Rotate the one-way system in S3 to gel under reduced pressure at 35-45°C, and add a certain amount of phosphate buffer solution for a period of time to complete the hydration. After ultrasonication for 5-15 minutes, place Freeze at -20°C for 8-15 hours, take it out and wait for it to melt and sonicate for 5-15 minutes to obtain proanthocyanidin liposome emulsion...

Embodiment 1

[0042] Embodiment one: the preparation method of a kind of PEG-modified proanthocyanidin liposome of the present embodiment is realized according to the following steps: one, take the hydrogenated lecithin of 0.1000g and the cholesterol of 0.0330g, be dissolved in 100ml with ether and dehydrated alcohol mixed solution 20ml In a beaker, ultrasonically shake at 50°C until completely dissolved to make a lipid solution. 2. Add 5g / L procyanidins and 6mL PBS to the lipid solution and sonicate for 5min to form a one-way system. 3. The single-phase system is decompressed at 40°C to make it gel-like, and supplemented with PBS to 20mL, rotary steamed for a period of time to make the hydration complete, after ultrasonication for 5-15min, put it in -20°C for 8-15h, take it out After it was melted, it was sonicated for 5-15 minutes to obtain the crude liposome. 4. The crude product is processed to obtain proanthocyanidin liposomes. 5. Mix the prepared proanthocyanidin liposome with 2% PE...

Embodiment 2

[0043] Example 2: The first difference of this embodiment is that in step 1, 0.1523g of hydrogenated lecithin and 0.1000g of cholesterol are placed in a beaker, and other steps are the same as in specific embodiment 1.

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Abstract

The invention belongs to the technical field of cosmetics, and discloses a preparation method of PEG (polyethylene glycol) modified proanthocyanidin liposomes. According to the invention, proanthocyanidin liposomes are prepared by taking hydrolecithins and cholesterols as lipid membrane wall materials, taking proanthocyanidins as coatings and adopting a reverse evaporation-freeze thawing method. In the liposome preparation process, the ratio of the lecithin to the cholesterol is 3-4, the ratio of the cholesterol to surface modifier is 1.5-3, the volume ratio of a water phase to organic solvent is 0.1-0.3, and the ratio of a drug to the liposome is 0.02-0.1; water bath ultrasonic treatment is performed for 10-20 minutes; and the liposomes are modified by adding 2% PEG1500. In the preparation method of PEG modified proanthocyanidin liposomes, the proanthocyanidin liposome encapsulation rate and storage stability are obviously improved, thereby providing a research basis for industrial production of liposomes.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and relates to a preparation method of PEG-modified proanthocyanidin liposome. Background technique [0002] Proanthocyanidins (PC) is a bioflavonoid with a special molecular structure, which can form two, three or even decamers according to the condensation of different amounts of catechin or epicatechin, among which dimer is the simplest Proanthocyanidins (OPC) are oligomers of catechin and epicatechin bonded through C4, C6 or C4, C8. Proanthocyanidins exist in many plants, and glucose and skin become the main source of proanthocyanidins. [0003] Proanthocyanidins are the most effective natural antioxidants for eliminating free radicals in the human body. Its anti-free radical oxidation ability is 50 times that of vitamin E and 20 times that of vitamin C. It also has cardiovascular protection, anti-radiation, anti-tumor, anti-inflammatory, anti-allergy, Skin care and beauty effects. Proan...

Claims

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Application Information

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IPC IPC(8): A61K8/14A61K8/86A61K8/49A61K8/55A61K8/63A61Q19/08A61Q19/00
CPCA61K8/14A61K8/498A61K8/553A61K8/63A61K8/86A61Q19/00A61Q19/08
Inventor 余倩郝秀青谭旭坤
Owner GUANGDONG UNIV OF TECH