Application and pharmaceutical composition of polysubstitution naphthalene derivative in preparing tumor angiogenesis resisting medicine
A naphthalene derivative and angiogenesis technology, applied in the field of biological activity application of compounds, can solve problems such as difficult development and unsatisfactory biological activity, and achieve the effects of enhancing sensitivity, inhibiting metastasis and recurrence, and reducing concentration
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Embodiment 1
[0020] Example 1: Effect test of NY-06D on endothelial cell migration induced by tumor cell culture medium
[0021] The effect of NY-06D on the migration of vascular endothelial cells induced by tumor cell culture medium was detected by wound healing method: Human umbilical vein endothelial cell line ECV304 cells in the logarithmic growth phase were cultured under normal conditions for 24 hours. Scratch the middle of the 24h well plate with a 0.5mm cell scraper, cut off the cell monolayer, and wash it with PBS twice to remove the scraped free cells.
[0022] The experiment was divided into negative control group, induction group and treatment group. In the induction group, 10% human breast cancer cell MCF-7 culture solution was added to the control group as a positive control; in the treatment group, 100 μL of 10% human breast cancer cell MCF-7 culture solution was added, and then NY-06D solution was added. (concentration 0.2 mg / mL, prepared from 1640 complete medium, filtere...
Embodiment 2
[0024] Example 2: Effect test of NY-06D on tumor angiogenesis in lung cancer
[0025] New Zealand rabbits were randomly divided into NY-06D group, ZOL group, NY-06D+ZOL group, blank control group, a total of 4 groups, 2 rabbits in each group. According to the dose in Table 1, each group was administered twice a day, with an interval of 12 hours, for 3 consecutive days, and 1 hour after the last intragastric administration, the carotid artery was bled, left overnight at 4°C, and centrifuged at 2500r / min for 25 minutes to separate the serum and mix it evenly to eliminate bacteria. Filter and inactivate, and freeze at -70°C for later use. Under the same conditions, the blank control serum was prepared by intragastric administration with an equal volume of normal saline.
[0026] Table 1 Dosage of each group
[0027]
[0028] Human lung adenocarcinoma cell line A549 and human umbilical vein endothelial cell line CRL-1730 were cultured in DMEM containing 10% fetal bovine serum...
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