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Application of rutin to preparation of drug for promoting mammal immune efficacy

A mammalian immune efficacy technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve problems such as unsatisfactory immune efficacy of vaccines

Inactive Publication Date: 2017-05-31
唐山滦牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides the application of rutin for the preparation of drugs for promoting immune efficacy of mammals, so as to solve the technical problem of unsatisfactory immune efficacy of vaccines in the prior art

Method used

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  • Application of rutin to preparation of drug for promoting mammal immune efficacy
  • Application of rutin to preparation of drug for promoting mammal immune efficacy
  • Application of rutin to preparation of drug for promoting mammal immune efficacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Vaccine immunization and administration of rutin

[0029] Group immunization of experimental mice: The experimental BALB / c mice were randomly divided into three groups, A, B, and C, with 10 mice per group. Group A was immunized with a commercial foot-and-mouth disease vaccine and then injected with rutin at a dose of 400 mg / kg. In aqueous solution, group B was immunized with the same vaccine as group A, and group C was a normal saline blank control group with subcutaneous multi-point immunization, 0.2 mL / group. Then the immune cell concentration, cytokine content and antibody level in the serum of the tested mice were detected.

[0030] 2. Detection of antibody concentration

[0031] (1) Antigen coating: Calculate the amount of coating antigen needed according to the number of coating holes, weigh a certain amount of synthetic peptide of viral antigen as coating antigen, dilute it to 5μg / mL with coating solution, and add to 96 wells In the plate, 100μL / well. Afterwards, ...

Embodiment 2

[0067] Take 60 pigs and randomly divide them into 3 groups with 20 pigs in each group, namely the synergistic immunization group, the vaccine group and the blank control group. Among them, the synergistic immunization group was first immunized with a commercial porcine adenovirus vaccine, and then an aqueous solution of rutin was injected at a dose of 400 mg / kg; the vaccine group was only immunized with a commercial porcine adenovirus vaccine; the blank control group was not immunized. After immunization, blood was collected every week to detect antibodies until 7 weeks. As shown in Table 4.

[0068] Table 4 Immunization methods and injection doses of different experimental groups

[0069] Group immunity Immunization method dose Synergistic immune group Vaccine + Rutin Intramuscular injection0.5ml Vaccine group vaccine Intramuscular injection0.5ml Blank control group / / /

[0070] On the 7th day after immunization, the adenovirus group I serotype 4 challenge was performed re...

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Abstract

The invention provides application of rutin to preparation of a drug for promoting mammal immune efficacy. According to the technical scheme, it is found through experimental means that rutin not only has the pharmacologic effects such as anti-oxidization and lipid metabolism regulation as disclosed in the prior art, but has a specific immune system regulating function. It is found through endosome experiments that after rutin injection, proliferation of T-lymphocytes in animal serum is improved remarkably, the content of CD4+ cells and CD8+ cells and the proportion of CD4+ cells and CD8+ cells in T-lymphocyte subpopulation are increased, the expression quantity of cell factors IFN-gamma and IL-4 is also increased, and therefore the immune system stimulating effect of rutin is verified. On this basis, the immunological enhancement effect, especially immune synergism with vaccines, of rutin for specific diseases is further studied. Experiment results show that by injecting a rutin solution after conducting adenovirus vaccine or foot-and-mouth disease vaccine immunity on animals, antibody titer can be increased remarkably, so that the immune effect of vaccines can be improved.

Description

Technical field [0001] The present invention relates to the technical field of veterinary biological products and vaccines, and further relates to a method for promoting immune efficacy, in particular to the application of rutin for preparing drugs for promoting immune efficacy of mammals. Background technique [0002] Vaccines refer to biological products made with various pathogenic microorganisms for vaccination. It uses pathogenic microorganisms (such as bacteria, rickettsiae, viruses, etc.) and their metabolites to be artificially attenuated, inactivated or genetically modified into an automatic immune preparation for the prevention of infectious diseases. The vaccine retains the characteristics of pathogenic bacteria that stimulate the animal's immune system. When the animal body comes into contact with this innocuous pathogen, the immune system will produce certain protective substances, such as immune hormones, active physiological substances, special antibodies, etc.; w...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K39/39A61P37/04
CPCA61K31/7048A61K39/39A61K2039/55511
Inventor 吴全忠吴尚昆郭常春靳慧君张守勇
Owner 唐山滦牧生物科技有限公司
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