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A kind of Suaeda salsa seed carotenoid extraction process

An extraction process, the technology of Suaeda salsa, applied in the field of plant extraction, achieves the effect of fast and effective extraction method, strong operability and simple process

Inactive Publication Date: 2020-01-31
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction method of carotenoids in Suaeda salsa seeds has never been reported

Method used

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  • A kind of Suaeda salsa seed carotenoid extraction process
  • A kind of Suaeda salsa seed carotenoid extraction process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A process for extracting carotenoids from Suaeda salsa seeds, comprising the following steps:

[0027] 1) Weigh 0.1 g of Suaeda salsa seeds respectively, and grind them fully in a mortar under liquid nitrogen.

[0028] 2) Determine the ratio of the extractant as petroleum ether: acetone = 1:1.

[0029] 3) Add 5 mL of extraction agent (petroleum ether: acetone volume ratio = 2:1), so that the solid-liquid ratio is 1:50.

[0030] 4) Ultrasonic extraction is performed for 10 minutes each time, the ultrasonic frequency is 600 W, and the ultrasonic temperature is 30 °C.

[0031] 5) Ultrasonic extraction is performed twice, and the ratio of material to liquid is controlled to be 1:50 for each ultrasonic extraction (after the first ultrasonic extraction, filter, and the obtained residue is subjected to the second ultrasonic extraction).

[0032] 6) Combine the extracts, centrifuge at 5000 rpm for 5 min, take the supernatant and dry it with nitrogen to obtain the Suaeda salsa...

Embodiment 2

[0034] A process for extracting carotenoids from Suaeda salsa seeds, comprising the following steps:

[0035] 1) Weigh 0.1 g of Suaeda salsa seeds respectively, and grind them fully in a mortar under liquid nitrogen.

[0036] 2) Determine the ratio of the extractant as petroleum ether: acetone = 1:1.

[0037] 3) Add 2, 3, 5 and 10 mL of extraction agent (petroleum ether: acetone volume ratio = 1:1) respectively, so that the solid-liquid ratio is 1:20, 1:30, 1:50, 1:100.

[0038]4) Ultrasonic extraction was performed for 8 minutes each time, the ultrasonic frequency was 580 W, and the ultrasonic temperature was 35 °C.

[0039] 5) Ultrasonic extraction is performed twice, and the ratio of solid to liquid is controlled to be 1:50 for each ultrasonic extraction.

[0040] 6) Combine the extracts, centrifuge at 5000 rpm for 5 min, take the supernatant and dry it with nitrogen to obtain the Suaeda salsa seed carotenoids. Then the volume was adjusted to 3 mL with acetone, and the l...

Embodiment 3

[0043] A process for extracting carotenoids from Suaeda salsa seeds, comprising the following steps:

[0044] 1) Weigh 0.1 g of Suaeda salsa seeds respectively, and grind them fully in a mortar under liquid nitrogen.

[0045] 2) Determine the ratio of the extraction agent as petroleum ether: acetone = 3:1, 2:1, 1:1, 1:2 and 1:4.

[0046] 3) Add 5 mL of extraction agent (petroleum ether: acetone volume ratios are 3:1, 2:1, 1:1, 1:2 and 1:4 respectively) to make the solid-liquid ratio 1:50.

[0047] 4) Ultrasonic extraction is performed for 8 minutes each time, the ultrasonic frequency is 580 W, and the ultrasonic temperature is 30 °C.

[0048] 5) Ultrasonic extraction is performed twice, and the ratio of solid to liquid is controlled to be 1:50 for each ultrasonic extraction.

[0049] 6) Combine the extracts, centrifuge at 5000 rpm for 5 min, take the supernatant and dry it with nitrogen to obtain the Suaeda salsa seed carotenoids. Then the volume was adjusted to 3 mL with a...

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Abstract

The invention discloses an extraction process of suaeda salsa seed carotenoid. The extraction process comprises the following steps: (1) sufficiently grinding suaeda salsa seeds under liquid nitrogen; (2) taking a mixed solvent of petroleum ether and acetone at the volume ratio ranging from (3 to 1) to (1 to 4) as an extracting agent; mixing the extracting agent with a sample ground in the step (1) so as to enable the material-liquid ratio of 1 to (45 to 80); (3) carrying out ultrasonic wave extraction on a solution mixed in the step (2), wherein the ultrasonic wave extraction time is 8min to 18min and the number of times of ultrasonic wave extraction is 2 to 3 times; combing extracting solutions and centrifuging; taking supernatant and drying with nitrogen to obtain the suaeda salsa seed carotenoid. By adopting the extraction process, the blank of the extraction process of the suaeda salsa seed carotenoid is filled and a relatively good and effective extraction method is screened and optimized; an experiment foundation is provided for deep processing and reutilization of suaeda salsa seeds and large-scale extraction of the carotenoid.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a process for extracting carotenoids from Suaeda salsa seeds. Background technique [0002] Globally, soil salinization and secondary salinization are widespread. China is one of the countries with a lot of saline-alkali land in the world, and the salinization phenomenon in coastal areas is particularly serious. Halophytes can grow and develop normally and complete their life cycle in a salinized environment, so they must have their specific salt-tolerant methods. Suaeda salsa is a typical dilute annual halophyte widely distributed in the Yellow River Delta region. Its dimorphic seeds show great differences in seed color, germination characteristics and salt tolerance. The color of dimorphic seeds is mainly determined by the seed coat, so the accumulation of pigment in the seed coat is of great significance in the adaptation of Suaeda salsa to the environment and the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00A61K36/185A61P39/06C09B67/54A23K20/179A23K50/75
CPCA61K36/185A61K2236/15A61K2236/333A61K2236/39C09B61/00C09B67/0096
Inventor 郭建荣索姗姗王宝山
Owner SHANDONG NORMAL UNIV
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