New method of replacing serum antibody monitoring by H5 subtype avian influenza egg yolk antibody

Abstract

The invention belongs to the technical field of egg yolk antibody monitoring, and in particular relates to a new method of replacing serum antibody monitoring by a H5 subtype avian influenza egg yolk antibody. The invention provides a method of replacing a serum sample by use of an egg yolk sample, and of replacing the serum antibody monitoring by use of an egg yolk solution extracted through a phosphate buffer solution and the egg yolk solution extracted through chloroform to perform the antibody monitoring. The method is characterized in that the blood sampling is unnecessary in the antibody monitoring process, the antibody monitoring process only needs to collect the eggs, cannot produce stress on the poultry and cannot influence the health and production performance of the poultry; the mixed solution H5 subtype avian influence antibody valence of two egg yolk treating solution can replace the serum antibody valence after being modified in the antibody monitoring process, the H5 subtype avian influence antibody is mainly used for the H5 subtype avian influenza antibody monitoring of the laying fowl and the breeding poultry, and further can be used for evaluating the nestling epidemic disease maternal antibody level so as to determine the first immune time.

Description

technical field [0001] The invention belongs to the technical field of egg yolk antibody monitoring, and in particular relates to a new monitoring method for H5 subtype avian influenza egg yolk antibody instead of serum antibody. Background technique [0002] H5 subtype avian influenza belongs to highly pathogenic avian influenza and is one of the most serious poultry infectious diseases. The level of antibody titer produced by immunization directly determines the quality of immunization and the success or failure of prevention and control. Since immunization is easily affected by many factors, such as vaccine factors, animal factors, feeding management factors, disease factors, etc., and the immune response of animals cannot be seen or touched, there is great uncertainty in the quality of immunity. Bring great difficulty to control. Antibody monitoring is the most direct and effective method to test the quality of immunization. Through antibody monitoring, scientific immu...

AI Technical Summary

Problems solved by technology

In recent years, there have been many methods that hope to replace serum antibody monitoring with egg yolk antibody monitoring, but there is a certain gap between the situation of egg yolk antibody and serum antibody, and the correlation between them is difficult to guarantee, which often leads to unreliable monitoring results

There are many existing technologies that have analyzed the correlation between the two, but the results are often limited to the numerical comparison of the measured potency, trying to summarize simple numerical rules from it, in order to make the monitoring of yolk antibody replace Serum antibodies, but this is not plausible

The determination of the titer of yolk antibody is the most critical point for yolk to replace serum. Compared with the titer determination technology of serum antibody, the technology for determining the titer of yolk antibody is very mature. Yolk antibody exists in both egg yolk and egg white, and is a heterogeneous liquid mixture. , it is difficult to ensure that all the antibodies in the yolk have participated in the antibody monitoring experiment in the existing technology. Depending on the actual method, it is often either less introduced or more antibodies are miscalculated. It is difficult to guarantee the obtained results with a single method. The reliability of the yolk antibody titer

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