A kind of preparation method of microbial inoculum for sustainable dredging and removing black and odorous water body
A technology for microbial inoculants and black and odorous water bodies, applied in chemical instruments and methods, biological water/sewage treatment, bacteria, etc., can solve the problems of being easily washed away, limited application places for microbial purifying inoculants, etc. Simple to use effects
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[0027] A method for preparing a microbial agent for sustainable dredging and removing black and odorous water bodies provided by the invention comprises the following steps:
[0028] Step 10, prepare plant culture medium: add 100-300 mesh ore powder for planting to the soil for planting green leafy plants, collect the grown green leafy plants and mix them with sterile purified water, and obtain plant medium by crushing and stirring , high-temperature disinfection, cooling to 30-40°C, and standby, wherein the carbon source concentration in the plant medium is 1g / L-13g / L, and the nitrogen source concentration is 0.1g / L-1g / L. The present invention uses ore powder to plant green leafy plants, which can enrich more trace elements during the growth process of the plants, and the content ratio of trace elements meets the needs of the plants themselves. As a plant medium, it is also more conducive to plant sources The cultivation and propagation of microorganisms do not need to add ad...
Embodiment 1
[0044] The raw material components used in this example are: 10.2 parts of ore powder for cultivation, 0.5 parts of plant culture medium, 0.3 parts of plant-derived microorganisms and 89.0 parts of sterile water.
[0045] Add 89.0 parts of sterile purified water for culture into the fermentation vessel, spread 3.4 parts of ore powder for culture on the bottom of the fermentation vessel, and let it stand for 15-30 minutes; add 0.5 parts of plant-derived culture medium and stir evenly; add 0.3 parts of Plant-derived microorganisms, stirred evenly, fermented at a constant temperature of 35°C for 24-48 hours, and aerated with oxygen every 15-30 minutes; the pH value of the liquid in the fermentation vessel was kept between 5.0-6.5.
[0046] Add 4.2 parts of ore powder for cultivation, ferment at a constant temperature of 30°C for 8 to 24 hours, aerate with oxygen every 30 to 60 minutes, and keep the pH value of the liquid in the fermentation vessel between 5.0 and 6.5.
[0047] Ad...
Embodiment 2
[0050] The raw material components used in this example are: 15.0 parts of ore powder for cultivation, 1.0 part of plant culture medium, 0.5 part of plant-derived microorganisms and 60.0 parts of sterile purified water for cultivation.
[0051] The difference from Example 1 is that 5.0 parts of ore powder for cultivation is spread on the bottom of the fermentation vessel, then 6.0 parts of ore powder for cultivation are added, and finally the remaining 4.0 parts of ore powder for cultivation are added.
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