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Fluorescent resonance probe and application and kit thereof

A fluorescence resonance and fluorescent probe technology, applied in the field of biochemistry, can solve problems such as the inability to clearly distinguish the degree of base sequence differences, and achieve broad application prospects, market value, high specificity and sensitivity

Active Publication Date: 2017-05-31
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned existing detection methods cannot clearly distinguish or accurately detect miRNA fragments or other types of RNA fragments or DNA with a small difference in base sequence (for example, only a few or one base difference). fragment

Method used

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  • Fluorescent resonance probe and application and kit thereof
  • Fluorescent resonance probe and application and kit thereof
  • Fluorescent resonance probe and application and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The fluorescence resonance probe provided in this embodiment includes a first fluorescent probe and a second fluorescent probe.

[0070] The first fluorescent probe has a first binding region and a first recognition region. The second fluorescent probe has a second binding region and a second recognition region.

[0071] The base sequence of the first binding area is reverse complementary to the base sequence of the second binding area, the end of the first binding area is marked with a first fluorescent group, and the end of the second binding area is marked with a second fluorescent gene.

[0072] The fluorescence spectrum of the first fluorescent gene overlaps with the excitation spectrum of the second fluorescent group. The first recognition region and the second recognition region are respectively reverse complementary to the adjacent first target region and second target region on the target nucleic acid fragment.

[0073] In this embodiment, the length of the f...

Embodiment 2

[0079] The structure of the fluorescence resonance probe provided in this example is basically the same as that in Example 1. The difference is: in this embodiment, the length of the first binding region is 8 bp, and the length of the second binding region is the same as that of the first binding region.

Embodiment 3

[0081] The structure of the fluorescence resonance probe provided in this example is basically the same as that in Example 1. The difference is: in this embodiment, the length of the first binding region is 9 bp, and the length of the second binding region is the same as that of the first binding region.

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Abstract

The invention provides a fluorescent resonance probe and an application and a kit thereof, and belongs to the field of biological chemistry. The fluorescent resonance probe is suitable for detection of a target nucleic acid segment and comprises a first fluorescence probe and a second fluorescence probe which can form a T-shaped structure together with the target nucleic acid segment. According to the T-shaped structure, fluorescent resonance energy transfer of a first fluorescence group on the first fluorescence probe and a second fluorescence group on the second fluorescence probe can be ensured. Qualitative or quantitative detection of the target nucleic acid segment is achieved through detecting the fluorescent resonance energy transfer efficiency. The fluorescent resonance probe has relatively high specificity and sensitivity, and the base sequence difference degree can be reduced to the target nucleic acid segment of one base for detection.

Description

technical field [0001] The present invention relates to the field of biochemistry, in particular to a fluorescent resonance probe and its application and kit. Background technique [0002] At present, the existing methods for detecting microRNA (miRNA) mainly include Northern blot analysis, microarray analysis and real-time fluorescent quantitative polymerase chain reaction. [0003] Among them, Northern blot analysis is a common method for detecting RNA based on hybridization, and it is one of the earliest methods for microRNA analysis. This method is simple and easy to operate, and most laboratories can operate it without additional capital investment and equipment update. Microarray analysis is also based on the principle of hybridization to detect microRNA. It analyzes and understands the expression regulation mechanism of microRNA and the expression of genes regulated by microRNA by measuring the expression level of microRNA in a specific process. [0004] Microarray ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 金宗文罗擎颖刘琳
Owner SHENZHEN INST OF ADVANCED TECH
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