Culture medium for Escherichia coli fermentation, preparation method and uses thereof
A technology of Escherichia coli and culture medium, which is applied to the culture medium and preparation field of Escherichia coli fermentation, can solve the problems of corrosion of stainless steel tank body by sodium chloride, high cost, low yield of recombinant protein, etc. The effect of yield
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0029]The method for preparing the above-mentioned medium provided by the present invention includes: preparing a buffer solution containing phosphate, dihydrogen phosphate, ammonium ions and other counter cations, adjusting the pH to 6.5-7.2, and sterilizing it for later use; preparing a glucose solution, filtering Sterilize for standby; prepare yeast extract solution and sterilize for standby; mix the above buffer solution, glucose solution and yeast extract solution to form a culture medium. Wherein the content of each component can reach the formulation amount of the culture medium. The above buffer solution and yeast extract solution can be sterilized at 121° C. and 0.15 MPa, then cooled to 40-50° C., and then mixed with the glucose solution.
[0030] The present invention provides a preferred method for preparing the above-mentioned culture medium. According to the ratio of adding 250mL of buffer, 100mL of glucose solution and 650mL of yeast extract solution to each 1L o...
Embodiment 1
[0034] The following takes the preparation of a medium with a final volume of 1 L as an example to describe in detail.
[0035] 1. Take sodium phosphate, potassium dihydrogen phosphate, (NH 4 ) 3 PO 4 The prepared concentrations are 0.02mol / L, 0.02mol / L and 0.03mol / L respectively, and the pH is adjusted to 6.5-7.2 as a buffer solution, which is then sterilized at 121°C and 0.15MPa for future use.
[0036] 2. Prepare 200 mL of 25% (mass / volume) glucose solution, sterilize at 115° C. and 0.15 Mpa for 30 minutes, and cool it down for later use.
[0037] 3. Weigh 2.5g of yeast extract powder, dissolve it with a small amount of distilled water, dilute to 650mL, sterilize at 121°C and 0.15MPa, and cool down for later use.
[0038] 4. When the buffer solution and yeast extract powder solution are cooled to 40-50°C, take 250mL of buffer solution, 100mL of glucose solution and 650mL of yeast extract powder solution and mix them on a clean bench to obtain a medium with a final volume...
Embodiment 2
[0040] The following takes the preparation of a medium with a final volume of 1 L as an example to describe in detail.
[0041] 1. Take potassium phosphate, sodium dihydrogen phosphate, (NH 4 ) 3 PO 4 The prepared concentrations are 0.01mol / L, 0.01mol / L and 0.02mol / L respectively, and the pH is adjusted to 6.5-7.2 as a buffer solution, which is then sterilized at 121°C and 0.15MPa for future use.
[0042] 2. Prepare 200 mL of 30% (mass / volume) glucose solution, sterilize at 115° C. and 0.15 Mpa for 30 minutes, and cool it down for later use.
[0043] 3. Weigh 1g of yeast extract powder, dissolve it with a small amount of distilled water, dilute to 650mL, sterilize at 121°C and 0.15MPa, and cool down for later use.
[0044] 4. When the buffer solution and yeast extract powder solution are cooled to 40-50°C, take 250mL of buffer solution, 100mL of glucose solution and 650mL of yeast extract powder solution and mix them on a clean bench to obtain a medium with a final volume o...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More