PCR detection primer for food-borne streptococcus hemolyticus virulence gene cps2J and kit of PCR detection primer

Abstract

The invention discloses a PCR detection primer for food-borne streptococcus hemolyticus virulence gene cps2J. The PCR detection primer comprises a sense primer cps2J-Fi, an antisense primer cps2J-Ri and an amplified product 1152bp. Accordingly, the inventor further assemblies a corresponding detection kit and a corresponding detection method. Specific detection and sensitivity detection verify that the PCR detection method is high in specificity, and can rapidly and accurately detect food-borne streptococcus hemolyticus carrying virulence gene from different intestinal bacteria; and moreover, the method is high in sensitivity, and can detect the lowest sample DNA concentrate which is 3.2*10<2> ng / ml. As all reagent components in the kit are confirmed through multiple tests to be high in repeatability during reaction, and the kit not only greatly shortens the time and reduces cost during detection, but also provides a novel technical means for clinic mass rapid and convenient detection.

Description

technical field [0001] The invention belongs to the technical field of detection of hemolytic streptococcus, in particular to a PCR detection primer and a kit thereof for the virulence gene cps2J of food-borne hemolytic streptococcus. Background technique [0002] The safety of animal-derived food has become a hot spot of public concern, and food-borne diseases caused by pathogenic pathogenic bacteria have become "potential killers" around people. In July 2005, major public health incidents occurred in Ziyang, Neijiang and other places in Sichuan, China. 208 people suffered from streptococcal disease and 38 died, involving 11 cities, 34 counties, 127 townships, and 197 villages (towns, street offices, etc.) Department), from May to July 2005, there were 11 cases of Streptococcus patients in Hong Kong, and 1 case died. At the International Food Safety Conference in April 2014, experts stated that the biggest food safety problem in the world is the problem of food-borne disea...

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Problems solved by technology

[0004] The routine detection method of pathogenic bacteria is to identify the phenotypic characteristics and biochemical indicators of the bacteria. The biochemical indicators for the identification of hemolytic streptococcus mainly include Gram staining, oxidase test, peroxidase test, CAMP test, V-P Reaction (Fergus-Pliscoll) product test, agglutination test, ELISA test, Lancefield's group-specific antigen group identification, etc., these methods have the characteristics of time-consuming, laborious, low sensitivity and poor specificity

At present, there are some automatic rapid bacterial identification systems for streptococcus identification, such as RAPID Strep strip, API rapid strep 32, API 20EVitek system, ATB rapid detection system, etc., but the results of automatic bacterial identification instruments for Streptococcus iniae are unstable, and Due to the limited number of template strains in the database of automatic bacterial identification, the key data of Streptococcus is still under study, so the application of this method is also limited

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