Freshwater fish clonorchis sinensis metacercaria infection colloidal gold rapid detection strip and making method thereof

Abstract

The invention belongs to the field of biological detection, and particularly relates to a freshwater fish clonorchis sinensis metacercaria infection colloidal gold rapid detection strip and a preparation method thereof. The strip comprises a support plate, and a sample pad, a gold label pad, a nitrocellulose membrane and an absorbent pad which are positioned on the surface of the support plate and arranged in sequence from a sample adding end, wherein the gold label pad comprises a colloidal gold labeled rabbit anti-freshwater fish polyclonal antibody; the nitrocellulose membrane is coated with a detection line T and a quality control line C. By adopting the detection strip, the problems of waste of time and labor, need of special instruments and equipment, high miss ratio, low efficiency, incapability of realizing non-invasive detection, incapability of realizing large-range popularization and the like in the conventional freshwater fish clonorchis sinensis metacercaria infection detection method are solved.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a colloidal gold rapid detection test strip for clonorchis sinensis metacercariae and a preparation method thereof. Background technique [0002] Clonorchis sinensis, also known as liver fluke, is a zoonotic parasitic disease with a large number of infected people and a wide range of epidemics in my country. Humans and animals are mainly infected with liver flukes by ingesting freshwater fish and shrimps that contain infecting live metacercariae. Adult worms mainly live in the hepatic bile ducts. On the one hand, they damage the bile duct epithelium or subcutaneous blood vessels through mechanical stimulation; on the other hand, the excretion products secreted by the worms can cause inflammation and other damage through the epithelial cells, and eventually cause suppurative cholangitis and obstructive cholelithiasis. , cholecystitis, pancreatitis, and even cause serious hepat...

AI Technical Summary

Problems solved by technology

This method requires high-end temperature control equipment, and has high requirements on the environment during operation, otherwise it is easy to pollute and cause false negatives

[0007] The above traditional methods for detecting metacercariae in freshwater fish have certain advantages but also have limitations, such as time-consuming and labor-intensive, detection is fatal to freshwater fish, and are not suitable for large-scale rapid detection in the field

[0010] At present, there is no similar research or product that uses colloidal gold immunochromatography to diagnose freshwater fluke infection-specific antibodies

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