Pharmaceutical composition and application thereof for treating heart failure
A technology of composition and drug, applied in the direction of drug combination, pharmaceutical formula, cardiovascular system diseases, etc.
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Embodiment 1
[0018] Example 1 The protective effect of the pharmaceutical composition on H9C2 cardiomyocytes
[0019] Place H9C2 cardiomyocytes in DMEM medium (containing 10% FBS) in an incubator (37°C, 5% CO 2 ) cultured in ), and subcultured after the cells were confluent to about 80%, the harvested cardiomyocytes were spread in a 24-well plate at a rate of 5000 / well, cultured in DMEM medium (containing 10% FBS) for 2 days, and then replaced with serum-free DMEM After the culture medium, the following tests were carried out:
[0020] (1) Effect of pharmaceutical composition on cardiomyocyte apoptosis induced by doxorubicin
[0021] The cells in each well were randomly divided into groups, specifically divided into a control group, a model group, and each administration group (5 wells in each group), and the final concentration of cells in the model group and each administration group was 10 -7 mol / L doxorubicin; the control group was added with the same amount of serum-free medium. Af...
Embodiment 2
[0031] Example 2 Effect of pharmaceutical composition on LAD ligated heart failure rat model
[0032] Model preparation: male SD rats, weighing 250±50g, 10 in each group. Anesthetized by intraperitoneal injection of 1% sodium pentobarbital (50mg / kg), the rats were fixed supine on a self-made mouse board. Endotracheal intubation, small animal ventilator room air ventilation, frequency 70 times / min, tidal volume 7-8ml. Continuous monitoring of ECG changes. Hair clipping and skin preparation on the chest, bury the electrocardiogram electrode needles under the skin of the left lower limb and right upper limb, cut the skin, subcutaneous tissue, chest muscles and fascia along the left fourth intercostal space, cut the fourth rib on the left side, and use blood vessels The forceps bluntly separated the intercostal muscles 3 cm long along the fourth rib, opened the chest cavity, stretched the ribs, and squeezed the heart out of the chest cavity with the thumb and four fingers of the...
Embodiment 3
[0043] Effect of embodiment 3 pharmaceutical composition on chronic heart failure rat model
[0044] Model preparation: take male SD rats, weighing 250±50g, 10 in each group. 1% pentobarbital anesthesia, abdominal incision of rats, arterial clips were placed above the right renal artery and left renal artery, so that the cross-sectional area of the abdominal aorta of the rats was reduced to 50-60% of the original, and the surviving rats were placed in animal cages The rats in the sham group were only operated without arterial clips.
[0045] Routine feeding of rats after the operation started administration after 12 weeks, and each pharmaceutical composition group was administered by intragastric administration, and the sham operation group and the model group were given an equal amount of normal saline (containing 1% CMC-Na), and after 4 weeks of administration, the rats were administered. Rats were tested.
[0046] The dosing regimen of each dosing group is as follows: ...
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