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Gel cutting type tubular anaerobic microorganism separating and culturing device and anaerobic microorganism separating and culturing method using device

An anaerobic microorganism, separation and culture technology, which is applied in the field of rubber-cutting tubular anaerobic microorganism separation and culture devices, can solve the problems of increasing the difficulty of cultivation, cumbersome and complicated work, limiting the application of anaerobic microorganisms, etc., and achieves a good success rate and cost. The effect of control, high segmentation flexibility, and good convenience

Active Publication Date: 2017-06-13
NINGBO URBAN ENVIRONMENT OBSERVATION & RES STATION NUEORS CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anaerobic bacteria are cultivated in anaerobic bags and anaerobic tanks. The work is cumbersome and complicated, the conditions are harsh, the success rate of cultivation is low, and it is difficult to meet the required standards.
The use of an incubator improves the success rate of cultivation to a certain extent and reduces the complexity of operation, but its high cost and high cost limit its application in anaerobic microorganism cultivation activities.
[0004] In addition, in the above-mentioned prior art, when cultivating some special bacteria such as methanogens, because of their harsh growth and survival conditions: methanogens are all obligate strict anaerobic bacteria, which are very sensitive to oxygen and will immediately decompose when exposed to oxygen. Inhibited, unable to grow, reproduce, or even die; methane bacteria grow very slowly, and it takes more than ten days or even dozens of days to grow colonies under artificial culture conditions. According to McCarty (McCarty), some methane bacteria need It takes seventy or eighty days to grow colonies, and even longer under natural conditions
Therefore, when this type of bacteria is cultivated, it is necessary to maintain a strict anaerobic environment and to have good environmental stability, which invisibly increases the difficulty of cultivating with anaerobic bags, anaerobic tanks, anaerobic boxes, etc.

Method used

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  • Gel cutting type tubular anaerobic microorganism separating and culturing device and anaerobic microorganism separating and culturing method using device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this embodiment, the rubber-cut tubular anaerobic microorganism separation and cultivation device includes a main body with a tubular culture chamber formed inside: when anaerobic microorganisms are cultivated, the culture medium is sealed in the culture chamber; after the culture is completed, the culture medium Extrude from one end of the culture chamber. When extruding, you can extrude all or part of it as needed. If you partially extrude, you can maintain an anaerobic culture environment after continuing to seal; and fill the vacant part with inert gas After deoxygenation, a relatively long-term anaerobic storage can be achieved.

Embodiment 2

[0025] In this embodiment, the only difference from Embodiment 1 is that the inner diameter of the tubular culture chamber on the main body is the same, that is, the whole culture chamber is a tubular structure with the same inner diameter, and the culture medium can also be relatively uniform in this type of culture chamber The medium gel structure formed by this scheme has good controllability, good colony distribution, easy to fill, good culture stability and observability, and is also convenient for screening and interception.

Embodiment 3

[0027] In this embodiment, the only difference from Embodiment 1 is that the inner diameter of the tubular culture cavity on the main body near the outlet end (referring to the section where the culture medium is extruded during use) is greater than the inner diameter farther from the outlet end, that is, It can be gradually enlarged from a distance from the outlet end to a distance from the outlet end (such as a cone shape or a trumpet shape or a stepped shape, etc.). This scheme is conducive to mass cultivation and is conducive to improving output efficiency.

[0028] Different from the above-mentioned embodiments, one end of the main body has an opening and a closed structure for sealing the culture chamber. This solution is further defined as that when one end of the main body is open, it is beneficial to maintain airtightness, thereby facilitating the maintenance of an anaerobic environment.

[0029] Different from the above-mentioned embodiments, both ends of the main bod...

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Abstract

The invention discloses a gel cutting type tubular anaerobic microorganism separating and culturing device and an anaerobic microorganism separating and culturing method using the device. The gel cutting type tubular anaerobic microorganism separating and culturing device comprises a main body, wherein a tubular culture chamber is formed in the main body; a culture medium is sealed in the culture chamber when culturing anaerobic microorganisms; the culture medium is thoroughly or partially extruded out of one end of the culture chamber after finishing culturing the anaerobic microorganisms. Through the scheme, the defects such as operation, environment control accuracy and observability and the like of conventional anaerobic culture appliances such as anaerobic culture bags and serum bottles and the like can be solved; the anaerobic microorganisms have higher controllability in the aspects such as strain culture, separation and culture environment control and the like; the culture of the anaerobic microorganisms is relatively efficient and convenient and is relatively high in success rate and cost controllability.

Description

technical field [0001] The invention relates to an anaerobic microorganism cultivation device and an anaerobic microorganism cultivation method using the device, in particular to a rubber-cut tubular anaerobic microorganism separation and cultivation device and an anaerobic microorganism cultivation method using the device. Background technique [0002] There are many kinds of anaerobic microorganisms in the living environment of human beings and the human body itself, and they are closely related to human beings. However, due to the difficulty in the isolation and pure culture of anaerobic microorganisms, the development of technologies and methods for studying anaerobic microorganisms is quite slow, resulting in human beings' understanding and utilization of anaerobic microorganisms falling far behind that of aerobic and facultative anaerobic microorganisms. research work. Until the past two decades, with the continuous improvement of anaerobic operation technology and th...

Claims

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Application Information

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IPC IPC(8): C12M1/26C12M1/24C12M1/00
CPCC12M23/06C12M33/04C12M33/12
Inventor 龙锡恩姚槐应
Owner NINGBO URBAN ENVIRONMENT OBSERVATION & RES STATION NUEORS CHINESE ACADEMY OF SCI