Culture medium for clostridium tetani

Abstract

The invention provides a culture medium for Clostridium tetani, which comprises the following ingredients in parts by weight: 8 parts of maltose, 6 parts of yeast powder, 2 parts of cucumber juice, 1 part of Folium Isatidis, 1 part of serine, 1 part of realgar, 1 part of black rice; on this basis, a specific amount of ferrous chloride, mulberry bark or distiller's grains can be added. On the basis of the common materials used in the culture medium, certain Chinese herbal medicines are added to the formula, which unexpectedly achieves an outstanding culture effect. It is especially suitable for the culture of Clostridium tetani, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Clostridium tetani. Background technique [0002] Bacillus tetani is the pathogenic bacterium that causes tetanus. It exists in large quantities in the intestinal tract of humans and animals. It pollutes the soil with feces and causes disease through wound infection. After the strain is cultured on ordinary agar plates for 24-48 hours, it can form irregular colonies with a diameter of more than 1mm, with a dense center and loose periphery, like feathery colonies, which are easy to migrate and spread on the surface of the medium. There are obvious hemolytic rings on the blood agar plate, cultured in blister medium, the broth is turbid, the meat residue is partially digested, slightly blackened, gas is produced, and methyl mercaptan (with a putrid smell) and hydrogen sulfide are produced. Generally, it does not ferment sugar, can liquefy gelatin, produc...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the technical defect of prior art, provides a kind of culture medium that is used for Clostridium tetani, to solve the technical problem that Clostridium tetani multiplication speed is relatively slow in the prior art