Unlock instant, AI-driven research and patent intelligence for your innovation.

Molecular markers for assisted selection of peach varieties containing recessive single gene hd and their breeding methods and applications

A technology of assisted selection and molecular markers, applied in the fields of molecular biology and peach genetics and breeding, can solve the problems of relying on tree retention time or fruit hardness identification, time-consuming breeding efficiency, and long growth cycle, so as to improve breeding efficiency and reduce breeding costs , the effect of simple method

Active Publication Date: 2019-08-30
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the pedigree method is mainly used in the breeding process of peach SH succulent varieties. The traditional pedigree method combines two groups of parents to select the optimal breeding variety from the segregated offspring. The SH succulent phenotype depends on the time of peach fruit staying on the tree or Identification of fruit hardness is difficult and the accuracy rate is very low
Peach is a perennial fruit tree with a long growth cycle. Since the SH genotype of the parents is not known, the parent selection is very blind. Usually, a large number of hybrids are used to select offspring, resulting in high cost, heavy workload, time-consuming and low breeding efficiency.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular markers for assisted selection of peach varieties containing recessive single gene hd and their breeding methods and applications
  • Molecular markers for assisted selection of peach varieties containing recessive single gene hd and their breeding methods and applications
  • Molecular markers for assisted selection of peach varieties containing recessive single gene hd and their breeding methods and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A molecular marker for assisted selection of peach cultivars containing a recessive single gene hd,

[0061] 1. PpYUC11 locus non-variation characteristic primers (detection of no CACTA transposon insertion):

[0062] Design the forward primer WILD-F at 1593bp upstream of the ATG of peach PpYUC11 wild-type gene, design the reverse primer WILD-R at 2515bp upstream of ATG, if there is a site variation, a 3514bp band will be amplified, if there is no site variation , the expected band of 942bp will be amplified, and the primers used are as follows:

[0063] The forward primer WILD-F primer sequence is 5'-ACATCAAACGGCAATCAATA-3',

[0064] The reverse primer WILD-R primer sequence is 5'-TTAGCCACGAAGGTTACTCA-3';

[0065] 2. The PpYUC11 site contains a variant characteristic primer (to detect the insertion of a CACTA transposon, and the peach PpYUC11 mutant gene has a CACTA transposon insertion at 2142bp upstream of the start codon ATG):

[0066] Design the forward primer C...

Embodiment 2

[0075] One of the methods for breeding SH fleshy peach varieties with assisted selection of molecular markers containing a recessive single gene hd peach variety described in Example 1, which comprises the following steps:

[0076] (1) Select the natural population as the material, extract its DNA, then perform PCR amplification with the primer sequences shown in the primer pairs WILD-F and WILD-R and CACTA-INS-F and CACTA-INS-R respectively, and The amplified products were separated and analyzed by electrophoresis, and the band patterns of the amplified results were as follows: figure 2 , 3 shown;

[0077] From figure 2 It can be obtained that the varieties that amplified the N50 band with WILD-F and WILD-R as primers include 'Zhongyoutao 16', 'yumyeong', 'Qinwang', 'Jingyu', 'Huayu', 'Xia Crisp', the others all amplified the N20 band;

[0078] From image 3 It can be obtained that using CACTA-INS-F and CACTA-INS-R as the primer pair to amplify the L50 band, there are ...

Embodiment 3

[0098] The second method for breeding SH fleshy peach varieties using the molecular markers described in Example 1 to assist in the selection of a recessive single-gene hd peach variety, the method comprises the following steps:

[0099] (1) Select the natural population as the material, extract its DNA, then perform PCR amplification with the primer sequences shown in the primer pairs WILD-F and WILD-R and CACTA-INS-F and CACTA-INS-R respectively, and The amplified products were separated and analyzed by electrophoresis, and the band patterns of the amplified results were as follows: figure 2 , image 3 shown;

[0100] From figure 2 available in China, the varieties that amplified the N50 band with WILD-F and WILD-R primers include 'Zhongyoutao 16', 'yumyeong', 'Qinwang', 'Jingyu', 'Huayu', ' Xiacui', and the N20 band was amplified in the others;

[0101] From image 3 It can be obtained that the L50 strips amplified by the primer pair of CACTA-INS-F and CACTA-INS-R co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker for assisted selection of peach varieties with single recessive gene hd, a breeding method using the molecular marker and application of the molecular marker; the molecular marker contains variation feature positive primer CACTA-INS-F and reverse primer CACTA-INS-R, and zero-variation-feature positive primer WILD-F and reverse primer WILD-R. The molecular marker above is used first to select peach varieties with single recessive gene hd, parents of genotype hdhd and parents of genotype Hdhd or high-quality parents with genotype HdHd are hybridized until single plants of SH pulp peach variety with genotype hdhd are selected from offspring; in conjunction with field phenotyping and ethylene measurement, it is possible to efficiently and directionally modify and cultivate SH pulp storage-tolerant varieties of peach; the molecular marker, the breeding method and the application of the molecular marker are of important practical significance to the breeding of novel peach varieties having good storage and transportation tolerance.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to the technical field of peach genetics and breeding, in particular to a molecular marker for auxiliary selection of peach varieties containing a recessive single gene hd, and a breeding method and application thereof. [0002] technical background [0003] Peach is a popular and popular fruit. According to the texture of the pulp, it can be divided into three types: solute, insoluble and hard (SH, stony hard). At present, the main peach varieties in China are solute peaches. Rapid softening not only greatly shortens the storage time of the fruit, but also the fruit is very susceptible to infection by pathogenic microorganisms after softening, causing a large amount of losses due to aging and rot every year, which is a key issue restricting the development of the peach industry. However, under the conditions of complete color change and high concentration of sugar accumulation, the hard p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895
Inventor 王志强曾文芳牛良潘磊丁义峰鲁振华崔国朝
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI