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A method for identifying honey varieties using non-targeted metabolomics technology

A variety and technology of honey, applied in the direction of scientific instruments, measuring devices, instruments, etc., can solve unscientific and difficult problems, achieve the effect of ensuring health and improving honey quality

Active Publication Date: 2018-07-17
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very difficult and unscientific to find a reliable marker to identify its botanical and geographical origin based on a class of compounds that were originally assumed subjectively.

Method used

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  • A method for identifying honey varieties using non-targeted metabolomics technology
  • A method for identifying honey varieties using non-targeted metabolomics technology
  • A method for identifying honey varieties using non-targeted metabolomics technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089]A kind of screening is used to distinguish the method for the characteristic marker of honey kind, described kind is litchi honey and acacia honey, and described method comprises the steps:

[0090] (1) A total of 210 honey samples were collected from Yunnan, Hainan, Fujian, Guangdong and Guangxi, the main producing areas of lychee honey in my country, and Liaoning and Shaanxi, the main producing areas of Yang Huai honey. Among them, there are 28 samples from Yunnan Province, 29 samples from Hainan Province, 29 samples from Fujian Province, 33 samples from Guangdong Province, 31 samples from Guangxi Zhuang Autonomous Region, 27 samples from Liaoning Province, and 33 samples from Shaanxi Province. The samples were stored in a well-ventilated sample storage room at 20°C and protected from light. In order to improve the representativeness of honey samples, 3-6 samples from each main production area are evenly mixed into one honey sample, and finally the number of honey samp...

Embodiment 2

[0105] A method for screening characteristic markers used to identify honey varieties, the varieties are litchi honey and acacia honey, the specific method is the same as in Example 1, the only difference is that the preparation of standard samples is different, and the analysis conditions are different. The conditions of this example As follows:

[0106] (1) Weigh 2.0±0.1g of each portion of honey and place it in a 10mL stoppered centrifuge tube, add 8mL of pure water, vortex for 5 minutes to mix, centrifuge at 8000rpm for 8 minutes to precipitate particulate impurities, and take the supernatant to obtain it;

[0107] (2) High performance liquid chromatography

[0108] Mobile phase: 0.2% formic acid water and 0.2% formic acid acetonitrile solution;

[0109] Elution conditions: 0–2.0min, 5%B; 2.0–7.0min, 5%–30%B; 7.0–13.0min, 30%–95%B; 13.0–18.0min, 95%B; 18.0–18.1min ,95%-5%B; 18.1-20.0min, 5%B;

[0110] Chromatographic column: Thermo Scientific Hypersil GOLD C-18column (2...

Embodiment 3

[0116] A method for screening characteristic markers used to identify honey varieties, the varieties are litchi honey and acacia honey, the specific method is the same as in Example 1, the only difference is that the preparation of standard samples is different, and the analysis conditions are different. The conditions of this example As follows:

[0117] (1) Weigh 3.0±0.1g of each portion of honey and place it in a 10mL centrifuge tube with stopper, add 10mL of pure water, vortex for 10min to mix, centrifuge at 10,000rpm for 7 minutes to precipitate particulate impurities, and take the supernatant to obtain the product;

[0118] (2) High performance liquid chromatography

[0119] Mobile phase: 0.05% formic acid water and 0.05% formic acid acetonitrile solution;

[0120] Elution conditions: 0–2.0min, 5%B; 2.0–7.0min, 5%–30%B; 7.0–13.0min, 30%–95%B; 13.0–18.0min, 95%B; 18.0–18.1min ,95%-5%B; 18.1-20.0min, 5%B;

[0121] Chromatographic column: Thermo Scientific Hypersil GOLD ...

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Abstract

The invention relates to a method for identifying the variety of honey by use of a non-target metabonomics technology. The method is characterized in that through a simple sample pre-treatment process, then lots of high-resolution mass spectrometric data are acquired through an ultra-high performance liquid chromatography-quadrupole / electrostatic field orbitrap high-resolution mass spectrometry method, interference caused by deviation and variation in experimental data through background deduction, chromatographic peak extraction, normalization, data conversion and data scaling, and after that, a real feature marker capable of distinguishing the variety of honey can be discovered and confirmed through principal component analysis, t-checkout, volcano plots and variable importance projection drawings. When the feature marker is utilized to identify the quality of honey, the identification results are objective and accurate. The method is used for not only source-tracing identification of the variety of honey but also variety source tracing and origin tracing identification of other agricultural products.

Description

technical field [0001] The invention relates to the technical field of honey variety identification, in particular to a method for screening characteristic markers for identifying honey varieties, and also to a method for using the characteristic markers to identify honey varieties. In particular, it relates to a method for screening characteristic markers used to identify acacia honey and lychee honey, and a method for identifying acacia honey and lychee honey. Background technique [0002] my country is a big beekeeping country in the world. The total number of bee colonies has reached 9.1 million, and the annual output of honey exceeds 450,000 tons. It is a big country in honey production, consumption and export. Modern scientific research has proved that honey contains nearly 200 kinds of endogenous components, glucose and fructose are the main components, followed by water, sucrose, minerals, vitamins, proteins and amino acids. Consumer interest in honey has been incre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 周金慧李熠问亚琴张文文陈兰珍张金振金玥赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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