Method for extracting thioglycoside compound from Semen Lepidium apetalum Willd and application thereof
The invention relates to a technology for extracting and extracting the Radix chinensis, which is applied in the field of extracting glucosinolates to achieve the effect of improving cell viability.
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Embodiment 1
[0020] Take 8 kg of the processed Tinglizi, add 10 times the weight of Tinglizi to extract three times, each time for 1.5 hours, and concentrate the extract under reduced pressure to 2000ml extract, the concentrated extract is 4 times the weight of Tingli The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifuged, concentrated to no alcohol smell (about 1500ml), go up Dianion HP-20 column (adsorption / desorption of highly porous styrene) Attached resin), first eluted with water to remove most of the polysaccharides, then eluted with 20% ethanol to obtain 20% ethanol eluted part A (71.2g), 20% ethanol eluted part A was discretely dissolved in water, centrifugally filtered, concentrated (about 30ml) by Toyopearl HW-40 column chromatography, eluted with water and 10% aqueous methanol successively to obtain water eluted fraction B1 (40.2g) and 10% methanol eluted fraction B2 (12.8g), component B2 was subjected to ODS...
Embodiment 2
[0022] Take 5kg of the processed Tinglizi, add water 10 times the weight of Tinglizi to extract three times, each time for 1.5h, and concentrate the extract under reduced pressure to 1500ml extract, and use 4 times of Tingli for the concentrated extract The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifuged, concentrated to no alcohol smell (about 1200ml), go up Dianion HP-20 post (adsorption / desorption of highly porous styrene) Attached resin), first eluted with water, then eluted with 20% ethanol to obtain 20% ethanol eluted part A (45.3g), 20% ethanol eluted part A was discretely dissolved in water, centrifugally filtered, and concentrated (about 25ml) Through Toyopearl HW-40 column chromatography, eluted with water and 10% aqueous methanol successively to obtain water-eluting component B1 (25.6g) and 10% methanol-eluting component B2 (9.1g), component B2 was reacted with ODS-18 Phase column chromatograph...
Embodiment 3
[0024] Take 10kg of the processed Tinglizi, add 10 times the weight of Tinglizi to extract three times, each time for 1.5h, the extract is concentrated under reduced pressure to 2500ml extract, and the concentrated extract is 4 times the weight of Tingli The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifugally filtered, concentrated to no alcohol smell (about 2000ml), the supernatant is centrifugally filtered, concentrated to no alcohol smell, put on Dianion HP -20 column (highly porous styrene adsorption / desorption resin), eluted with water first, then eluted with 20% ethanol to obtain 20% ethanol elution site A (88.9g), 20% ethanol elution site A Dissolved in water, centrifugally filtered, concentrated (40ml) and passed through Toyopearl HW-40 column chromatography, followed by elution with water and 10% aqueous methanol to obtain water-eluted fraction B1 (51.0 g) and 10% methanol-eluted fraction B2 (16.3g...
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