Method for extracting thioglycoside compound from Semen Lepidium apetalum Willd and application thereof

The invention relates to a technology for extracting and extracting the Radix chinensis, which is applied in the field of extracting glucosinolates to achieve the effect of improving cell viability.

Inactive Publication Date: 2017-06-20
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no related reports on the separation of glucosinolates from the water extract of Tinglizi and the study of its application.

Method used

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  • Method for extracting thioglycoside compound from Semen Lepidium apetalum Willd and application thereof
  • Method for extracting thioglycoside compound from Semen Lepidium apetalum Willd and application thereof
  • Method for extracting thioglycoside compound from Semen Lepidium apetalum Willd and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 8 kg of the processed Tinglizi, add 10 times the weight of Tinglizi to extract three times, each time for 1.5 hours, and concentrate the extract under reduced pressure to 2000ml extract, the concentrated extract is 4 times the weight of Tingli The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifuged, concentrated to no alcohol smell (about 1500ml), go up Dianion HP-20 column (adsorption / desorption of highly porous styrene) Attached resin), first eluted with water to remove most of the polysaccharides, then eluted with 20% ethanol to obtain 20% ethanol eluted part A (71.2g), 20% ethanol eluted part A was discretely dissolved in water, centrifugally filtered, concentrated (about 30ml) by Toyopearl HW-40 column chromatography, eluted with water and 10% aqueous methanol successively to obtain water eluted fraction B1 (40.2g) and 10% methanol eluted fraction B2 (12.8g), component B2 was subjected to ODS...

Embodiment 2

[0022] Take 5kg of the processed Tinglizi, add water 10 times the weight of Tinglizi to extract three times, each time for 1.5h, and concentrate the extract under reduced pressure to 1500ml extract, and use 4 times of Tingli for the concentrated extract The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifuged, concentrated to no alcohol smell (about 1200ml), go up Dianion HP-20 post (adsorption / desorption of highly porous styrene) Attached resin), first eluted with water, then eluted with 20% ethanol to obtain 20% ethanol eluted part A (45.3g), 20% ethanol eluted part A was discretely dissolved in water, centrifugally filtered, and concentrated (about 25ml) Through Toyopearl HW-40 column chromatography, eluted with water and 10% aqueous methanol successively to obtain water-eluting component B1 (25.6g) and 10% methanol-eluting component B2 (9.1g), component B2 was reacted with ODS-18 Phase column chromatograph...

Embodiment 3

[0024] Take 10kg of the processed Tinglizi, add 10 times the weight of Tinglizi to extract three times, each time for 1.5h, the extract is concentrated under reduced pressure to 2500ml extract, and the concentrated extract is 4 times the weight of Tingli The volume concentration is 80% ethanol alcohol precipitation, alcohol precipitation 5 times, the supernatant is centrifugally filtered, concentrated to no alcohol smell (about 2000ml), the supernatant is centrifugally filtered, concentrated to no alcohol smell, put on Dianion HP -20 column (highly porous styrene adsorption / desorption resin), eluted with water first, then eluted with 20% ethanol to obtain 20% ethanol elution site A (88.9g), 20% ethanol elution site A Dissolved in water, centrifugally filtered, concentrated (40ml) and passed through Toyopearl HW-40 column chromatography, followed by elution with water and 10% aqueous methanol to obtain water-eluted fraction B1 (51.0 g) and 10% methanol-eluted fraction B2 (16.3g...

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Abstract

The invention relates to a method for extracting a thioglycoside compound from Semen Lepidium apetalum Willd and application thereof, and can effectively exploit the new action of Semen Lepidium apetalum Willd. The technical scheme is as follows: the molecular formula of the thioglycoside compound is C14H19NO6S[M+H]+m / z 330.1013.According to the invention, the content of the thioglycoside compound obtained from Semen Lepidium apetalum Willd through separation is high; and the thioglycoside compound has a certain protection effect on a hydrogen peroxide-impaired rat myocardial cell H9c2 and can obviously improve the activity of the cell, thereby providing a new pathway for preparing myocardial protection drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for extracting glucosinolate compounds from Tinglizi chinensis and its application. Background technique [0002] Tinglizi was first recorded in "Shen Nong's Materia Medica", listed as a low-grade herbal medicine, and is a commonly used Chinese herbal medicine. The "Tinglizi" included in the 2015 edition of "Chinese Pharmacopoeia" has a north-south distinction. Among them, the dry mature seeds of the cruciferous plant Lepidium apetalum Willd. .) The dry mature seeds of Webbex Prantl. are commonly called "Nan Ting Li Zi". The medicine tastes pungent and bitter, and is very cold in nature. It returns to the lung and bladder meridians, and has the effects of venting and relieving asthma, promoting water and reducing swelling. There are many studies on Fructus Tingli, and its chemical components mainly include flavonoids, glucosinolates, isothiocyanates, etc., while Fructus Tingli ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08A61P9/00A61P9/10
CPCC07H15/18C07H1/08
Inventor 郑晓珂李孟杨方方冯卫生张志广张靖柯吕锦锦赵璇
Owner HENAN UNIV OF CHINESE MEDICINE
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