Birch bpspl9 gene and its encoded protein and application

Abstract

The invention relates to a BpSPL9 gene of betula platyphylla Suk., an encoding protein thereof and applications of the gene and discloses the BpSPL9 gene of betula platyphylla Suk., the encoding protein thereof and the applications of the gene. The objective of the invention is to provide the BpSPL9 gene of betula platyphylla Suk., the encoding protein thereof and the applications of the gene. The nucleotide sequence of the gene is shown as SEQ ID NO:1 in a sequence table, and an amino sequence is shown as SEQ ID NO:2 in the sequence table. The gene can enhance adversity stress resistance of plants. An agrobacterium-tumefaciens-mediated leaf disc transformation method is utilized to perform genetic transformation of the betula platyphylla Suk., obtained transgenic betula platyphylla Suk. plants are subjected to salt resistance and drought tolerance analysis, and results show that the gene can enhance salt resistance and drought tolerance of the betula platyphylla Suk.. Through the gene, the encoding protein and the applications, a novel BpSPL9-transgenic betula platyphylla Suk. variety that is salt-resistant and drought-tolerant can be cultivated. The gene, the encoding protein and the applications are applied in the field of forest tree molecular breeding.

Description

technical field [0001] The invention relates to birch BpSPL9 gene and its encoded protein and application. Background technique [0002] Transcription factors (transcription factors, TFs), also known as trans-acting factors, refer to those protein molecules that specifically bind to cis-acting elements in the promoter region of eukaryotic genes, or bind to the structural characteristics of known DNA binding domains, It has the function of activating or inhibiting gene transcription effect. The number of transcription factors in higher plants is large, there are many types, and the transcription regulation is relatively complex. The research on transcription factors has attracted more and more attention. At present, a large number of transcription factors related to the regulation of drought, high salinity, low temperature, hormones, pathogenic response and growth and development have been isolated from higher plants. [0003] A typical plant transcription factor generally ...

AI Technical Summary

Problems solved by technology

However, different transcription factors may lack a certain functional region, such as a specific DNA binding domain or a transcriptional regulatory domain, through which the transcription factor enters the nucleus at a specific time and interacts with the cis-acting element of the promoter or with other transcription factors. Interaction of functional domains to regulate transcriptional expression of genes

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