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Syzygium buxifolium sprout rapid in vitro culture method

A cultivating method and a technique for rhizoma rhizoma, applied in the field of plant reproduction, can solve the problems of slow growth and low bud proliferation coefficient, and achieve the effects of large yield of sub-generation buds, simple operation process and lower production cost.

Inactive Publication Date: 2017-06-27
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method of tissue culture and propagation of red nan shoots with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the process of red nan tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Red Phoebe in the same year as explants;

[0026] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0027] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+6...

Embodiment 2

[0033] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Red Phoebe in the same year as explants;

[0034] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WP...

Embodiment 3

[0041] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Red Phoebe in the same year as explants;

[0042] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WP...

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PUM

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Abstract

The invention discloses a syzygium buxifolium sprout rapid in vitro culture method which comprises the processes of explant selection, explant treatment, initial bud induction culture, enrichment culture, strong bud culture and rooting culture, a semi-lignified current-year syzygium buxifolium sprout which grows robustly is collected and taken as an explant, after trimming and sterilization disinfection treatment of the explant, the explant is inoculated to an initial bud induction culture medium to obtain an initial bud, then the initial bud is inoculated to the initial bud induction culture medium, a cluster bud is formed after 4-5 cycles of enrichment culture, the cluster bud is inoculated to a strong bud culture medium for culture, and finally a single bud with the height larger than or equal to 2 cm is inserted into a rooting culture medium for induction rooting. According to the syzygium buxifolium sprout rapid in vitro culture method, the seedling culture period of the syzygium buxifolium is shortened, the seedling culture materials are saved, the problems that many materials are needed for seedling culture, the period is long and the like in traditional seedling culture methods are solved, the production cost is reduced greatly, the production efficiency is improved, and the method has better economic benefits, social benefits and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a method for rapid in vitro cultivation of red nan sprouts. Background technique [0002] Red Nan (Syzygium buxifolium Hook. et Arn.), belonging to Myrtaceae, is a shrub or small tree, with ridged shoots and dark brown after drying. Leaf blade leathery, broadly elliptic to elliptic, sometimes broadly obovate, apex round or blunt, sometimes with blunt apex, base broadly cuneate or blunt, upper part dark brown after drying, matte, lower part light-colored, with glandular spots, Lateral veins numerous and dense, inconspicuous above, slightly protruding below; styles equal to stamens. Fruit spherical, 5-7 mm in diameter. The flowering period is June-August. Produced in Anhui, Zhejiang, Taiwan, Fujian, Jiangxi, Hunan, Guangdong, Guangxi, Guizhou and other provinces and regions in China. Born in low mountain forests or shrubs. Red Nan likes light, is also resistant to shad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 唐春艳陈素云
Owner 唐春艳
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