Water chestnut shell flavone extract with bacteriostatic activity, preparation method and application thereof

A technology for bacteriostatic activity and flavonoids is applied in the field of water chestnut shell flavonoids extract and its preparation, which can solve the problems of few research reports and patents on the water chestnut shell flavonoid extract, achieve simple preparation process, avoid the use of organic reagents, time shortening effect

Inactive Publication Date: 2017-06-27
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many reports and patents about water chestnut and its application, but there are few research reports and patents about water chestnut shell flavonoids extract

Method used

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  • Water chestnut shell flavone extract with bacteriostatic activity, preparation method and application thereof
  • Water chestnut shell flavone extract with bacteriostatic activity, preparation method and application thereof
  • Water chestnut shell flavone extract with bacteriostatic activity, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of water chestnut shell flavonoids extract:

[0046] 1) Extraction: Remove the dried and crushed water chestnut shells that have passed through a 40-mesh sieve and mix them with 50% ethanol solution at a ratio of 1:20, ultrasonically treat for 2 minutes, then extract at a constant temperature of 55°C for 3 hours, then separate the extract, repeat the above operation for extraction twice, Combine the extracts, filter, and concentrate under reduced pressure at 45°C until there is no ethanol to obtain the crude extract of flavonoids from water chestnut shell;

[0047] 2) Adsorption: adjust the concentration of water chestnut shell flavonoids extract to 5.0 mg / mL, and perform AB-8 macroporous adsorption resin column chromatography; the adsorption flow rate is 0.5 times the column volume / h, and the adsorption volume is 3 times the column volume;

[0048] 3) Elution: elution was carried out with an elution flow rate of 0.8 mL / min and 70% ethanol solution as the el...

Embodiment 2

[0051] Preparation of water chestnut shell flavonoids extract:

[0052] 1) Extraction: remove the dried and crushed water chestnut shells that have passed through a 40-mesh sieve and mix them with 80% ethanol solution at a ratio of 1:30, ultrasonically treat for 1 min, then extract at a constant temperature of 50°C for 2 hours, then separate the extract, and repeat the above operation for 3 times. Combine the extracts, filter, and concentrate under reduced pressure at 50°C until there is no ethanol to obtain the crude extract of flavonoids from water chestnut shell;

[0053] 2) Adsorption: adjust the concentration of water chestnut shell flavonoids extract to 3.0mg / mL, and perform AB-8 macroporous adsorption resin column chromatography; the adsorption flow rate is 1 times the column volume / h, and the adsorption volume is 4 times the column volume,

[0054] 3) Elution: elution is carried out with an elution flow rate of 1.8 mL / min and 60% ethanol solution as the eluent, and the...

Embodiment 3

[0058] Preparation of water chestnut shell flavonoids extract:

[0059] 1) Extraction: Remove the dried and crushed water chestnut shells that have passed through a 40-mesh sieve and mix them with 65% ethanol solution at a ratio of 1:25, ultrasonically treat for 2 minutes, then extract at a constant temperature of 60°C for 2 hours, then separate the extract, and repeat the above operation for 3 times. Combine the extracts, filter, and concentrate under reduced pressure at 50°C until there is no ethanol to obtain the crude extract of flavonoids from water chestnut shell;

[0060] 2) Adsorption: Adjust the concentration of water chestnut shell flavonoids extract to 3.0mg / mL, and perform AB-8 macroporous adsorption resin column chromatography; the adsorption flow rate is 0.8 times the column volume / h, and the adsorption volume is 1.5 times the column volume,

[0061] 3) Elution: elution was carried out with an elution flow rate of 0.8 mL / min and 70% ethanol solution as the eluent...

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Abstract

The invention belongs to the technical field of natural product extraction and specifically relates to a water chestnut shell flavone extract with bacteriostatic activity, a preparation method and an application thereof. According to the invention, the water chestnut shell flavone is extracted under lower temperature, the tissue cell walls of the water chestnut shell are ultrasonically treated and broken and the macroporous resin column chromatography is adopted for purifying, so that the bacteriostatic activity of the extract is remained as far as possible; under the enriching effect of the macroporous resin, the purity of the water chestnut shell flavone is high; the acquired water chestnut shell flavone extract has an obvious antibacterial effect; the technical scheme is low in dosage of organic reagent, is safe and nontoxic and can be used for developing natural bacteriostatic agent.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to an extract of water chestnut shell flavonoids with antibacterial activity, a preparation method and application thereof. Background technique [0002] Water chestnut is not only a traditional food but also a traditional medicinal material. It has health effects such as anti-tumor and enhancing immunity. Water chestnut is widely planted in the southern provinces of my country, water chestnut shells are generally discarded as waste, and a large amount of functional substances are not utilized, resulting in waste of resources and environmental pollution. Water chestnut shell is rich in effective substances such as polyphenols, polysaccharides, flavonoids and alkaloids, and the preparation and development of functional substances have great market prospects. Among them, the flavonoid extract of water chestnut shell has good anti-oxidation and antibacte...

Claims

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Application Information

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IPC IPC(8): A23B4/22
CPCA23B4/22
Inventor 王丽梅王宏勋易阳闵婷侯温甫艾有伟侯莉莉
Owner WUHAN POLYTECHNIC UNIVERSITY
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