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Double aptamer modified mesoporous silica targeted drug-loading nanoparticle

A technology of mesoporous silica and drug-loaded nanometers, applied in the field of biomedicine, can solve the problems of poor specificity, increase the ability of mesoporous silica nanoparticles to recognize target cells, etc., and achieve the effect of inhibiting tumor metastasis.

Inactive Publication Date: 2017-06-27
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the shortcomings of existing targeted drug delivery carriers such as poor specificity, the present invention provides a pair of aptamer-modified mesoporous silica targeted drug-loaded nanoparticles, which can utilize the specific recognition ability of two aptamers to synergistically Increase the recognition ability of mesoporous silica nanoparticles to target cells, so as to achieve the purpose of accurate drug delivery

Method used

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  • Double aptamer modified mesoporous silica targeted drug-loading nanoparticle
  • Double aptamer modified mesoporous silica targeted drug-loading nanoparticle
  • Double aptamer modified mesoporous silica targeted drug-loading nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Take 2.0 g of cetyltrimethylammonium chloride (CTAC) and 0.1 g of triethanolamine (TEA), dissolve them in 20 mL of ultrapure water, stir in an oil bath at 95 °C for 1 h, and then add 1.5 g of mL of tetraethylsilane (TEOS), continue to stir in a 95°C oil bath for 1 h; stop heating, and after the reaction liquid is cooled to room temperature, centrifuge at 15,000 rpm for 10 min, remove the supernatant, and use anhydrous Wash with ethanol 3 times to remove reaction residues, then add the washed solid matter into 20 mL sodium chloride-methanol solution (1 g sodium chloride per 100 mL methanol), stir at room temperature for 4 h to remove CTAC, The resulting solid material was freeze-dried to obtain white solid MSN-OH.

Embodiment 2

[0037] Dissolve 100 mg of the obtained MSN-OH and 2.5 mL of 3-aminopropyltriethoxysilane (APTES) in 20 mL of absolute ethanol, stir at room temperature for 12 h, then centrifuge the sample solution at 15,000 rpm for 10 min, and pipette In addition to the supernatant, the obtained precipitate was washed three times with absolute ethanol and ultrapure water to remove the reaction residue, and then the obtained solid matter was freeze-dried to obtain MSN-NH 2 . The resulting MSN-NH 2 The TEM image of figure 1 .

Embodiment 3

[0039] Add 7 nmol of carboxy-modified E-AP and (or) C-AP, 8 mg of EDC, 8 mg of NHS to 5 mL of ultrapure water, stir for 15 min and then add 10 mg of MSN-NH 2 , and continued to stir for 12 h; then the reaction mixture was centrifuged at 15,000 rpm for 10 min, the supernatant was removed, and dried to obtain E-MSN, C-MSN, and EC-MSN, respectively.

[0040] The resulting MSN-NH 2 Compared with the particle size distribution of EC-MSN figure 2 .

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Abstract

The invention discloses a double aptamer modified mesoporous silica targeted drug-loading nanoparticle and preparation and application thereof. The targeted drug-loading nanoparticle is composed of mesoporous silica nanoparticles, anti-cancer drugs coated and carried in the pore channels of the mesoporous silica nanoparticles and two types of nucleic acidaptamers modified on the surfaces of the mesoporous silica nanoparticles and capable of specifically identifying specific proteins on the surfaces of cancer cells. The targeted drug-loading nanoparticle can identify tumor sites and cancer cells circulated in blood in a targeted mode and can be used for preparation of drugs for preventing and treating tumor metastasis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a double-aptamer-modified mesoporous silicon dioxide targeted drug-loaded nanoparticle and its preparation and application. Background technique [0002] Cancer is one of the main causes of threats to human health today, and it is still difficult to cure. More than 90% of cancer patients die from tumor metastasis. Tumor metastasis is considered to be the formation of new metastatic lesions due to the shedding of tumor cells from tumor in situ and reaching new sites through blood circulation. Therefore, the development of targeted nanocarriers for tumor metastasis can effectively inhibit tumor metastasis, thereby reducing the number of cancer deaths. [0003] Circulating Tumor Cells (CTCs) in the blood have many specific proteins on the surface, such as EpCAM, CD44, HER2, etc., but these proteins are not expressed or expressed at a very low level in normal cells....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/54A61K31/704A61P35/04B82Y5/00
CPCA61K31/704B82Y5/00
Inventor 高瑜解晓东黎凤乔贾力
Owner FUZHOU UNIV
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