Method for pretreating hibiscus sabdariffa linn pigment extracting solution before on-column adsorption and purification
A technology of extract and roselle, which is applied in the field of pretreatment before column adsorption and purification of roselle pigment extract, which can solve the problems of time-consuming, product purity decline, waste of water, etc., to avoid clogging the resin column and to reduce manufacturing costs , Reduce the effect of cleaning frequency
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Embodiment 1
[0017] Below in conjunction with technical scheme, describe concrete production steps of the present invention in detail:
[0018] (1) Pigment extraction: extract 50 g of dried roselle calyx and 750 mL of distilled water (mass volume ratio 1:15), extract in a constant temperature water bath at 50°C for 2 hours, and pour out the extract.
[0019] (2) Filtration: filter the extract with ordinary filter paper, and collect the filtrate.
[0020] (3) Quartz sand filtration and resin adsorption of pigments: the size of the quartz sand filter is 1×15 cm, and half of the height of the column is added to the quartz sand. Take an appropriate amount of AB-8 adsorption resin, soak it in distilled water first, wash it with distilled water until the liquid is clear, then soak it with 95% ethanol for 4 hours, wash it with distilled water until it is not cloudy, and pour it into a 1×100cm adsorption column. Connect the quartz sand filter and the adsorption column in series, and connect them ...
Embodiment 2
[0023] Same as Example 1, except that in step (1), 0.1 g of pectinase is added together with the material, and the enzyme activity of pectinase is 30,000 U / g.
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