Preparation method of plasmodium pf/pan detecting test paper

A technology for detecting test strips and malaria parasites, applied in measuring devices, resistance to vector-borne diseases, instruments, etc., can solve the problems of time-consuming and laborious, misdiagnosis and missed diagnosis, and achieve the effect of accurate results and fast diagnosis

Inactive Publication Date: 2017-06-30
SUZHOU WANMUCHUN BIOLOGICAL TECH
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The etiological diagnosis (microscopic blood film examination) microscopic examination in the etiological diagnosis technology of malaria is time-consuming and laborious, and requires professional operation. It is p

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of plasmodium pf/pan detecting test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] (1) Containers and materials

[0024] Container equipment: beakers, measuring cylinders, volumetric flasks, glass rods, magnetic stirrers, electronic balances, pipette guns, film spraying equipment, cutting machines, strip cutting machines, sealing machines, shell pressing machines, pH meters, blast drying box.

[0025] Reagent accessories: chloroauric acid, trisodium citrate, K 2 CO 3 , sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, Tris (trishydroxymethylaminomethane), HCl, BSA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of plasmodium pf/pan detecting test paper. The preparation method comprises the following steps of: separately performing metal spraying to a glass cellulose membrane by means of immunogold marked by a plasmodium falciparum lactic dehydrogenase monoclonal antibody and a plasmodium lactic dehydrogenase monoclonal antibody to obtain a pf gold pad and a pan gold pad; separately marking lines on a polyvinyl chloride bottom plate adhered with a nitrocellulose membrane by a plasmodium falciparum lactic dehydrogenase monoclonal antibody detection line coating liquid, a plasmodium lactic dehydrogenase monoclonal antibody detection line coating liquid and a control line coating liquid to obtain the polyvinyl chloride bottom plate with the dotted membrane; and assembling a sample pad, the gold pads, the polyvinyl chloride bottom plate with the dotted membrane and water absorbing paper together and performing cutting to obtain the detecting test paper. The detecting test paper obtained by the method disclosed by the invention can judge whether a blood sample contains plasmodium pf/pan or not by manual operation and reading by naked eyes by virtue of an immune directed flow chromatographic technique, is fast to diagnose and accurate in result, and does not damage the body of a testee.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a preparation method of a malaria parasite pf / pan detection test paper. Background technique [0002] Malaria is a vector-borne infectious disease caused by the infection of Plasmodium through the bite of Anopheles mosquito or the transfusion of the blood of a person with Plasmodium. It ranks first in the world's deadly parasitic diseases. There are four types of Plasmodium parasites in the human body, namely Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. In my country, mainly Plasmodium vivax and Plasmodium falciparum, the other two are rare, and some cases imported from abroad have occasionally been seen in recent years. Different Plasmodium species cause Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. The main manifestations of malaria are periodic and regular attacks, with chi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/577G01N33/569
CPCG01N33/56905G01N33/577G01N2333/445Y02A50/30
Inventor 马军丁锦勤黄政
Owner SUZHOU WANMUCHUN BIOLOGICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap