Preparation method of plasmodium pf/pan detecting test paper

Abstract

The invention discloses a preparation method of plasmodium pf / pan detecting test paper. The preparation method comprises the following steps of: separately performing metal spraying to a glass cellulose membrane by means of immunogold marked by a plasmodium falciparum lactic dehydrogenase monoclonal antibody and a plasmodium lactic dehydrogenase monoclonal antibody to obtain a pf gold pad and a pan gold pad; separately marking lines on a polyvinyl chloride bottom plate adhered with a nitrocellulose membrane by a plasmodium falciparum lactic dehydrogenase monoclonal antibody detection line coating liquid, a plasmodium lactic dehydrogenase monoclonal antibody detection line coating liquid and a control line coating liquid to obtain the polyvinyl chloride bottom plate with the dotted membrane; and assembling a sample pad, the gold pads, the polyvinyl chloride bottom plate with the dotted membrane and water absorbing paper together and performing cutting to obtain the detecting test paper. The detecting test paper obtained by the method disclosed by the invention can judge whether a blood sample contains plasmodium pf / pan or not by manual operation and reading by naked eyes by virtue of an immune directed flow chromatographic technique, is fast to diagnose and accurate in result, and does not damage the body of a testee.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a preparation method of a malaria parasite pf / pan detection test paper. Background technique [0002] Malaria is a vector-borne infectious disease caused by the infection of Plasmodium through the bite of Anopheles mosquito or the transfusion of the blood of a person with Plasmodium. It ranks first in the world's deadly parasitic diseases. There are four types of Plasmodium parasites in the human body, namely Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. In my country, mainly Plasmodium vivax and Plasmodium falciparum, the other two are rare, and some cases imported from abroad have occasionally been seen in recent years. Different Plasmodium species cause Plasmodium vivax, Plasmodium malariae, Plasmodium falciparum and Plasmodium ovale. The main manifestations of malaria are periodic and regular attacks, with chi...

AI Technical Summary

Problems solved by technology

The etiological diagnosis (microscopic blood film examination) microscopic examination in the etiological diagnosis technology of malaria is time-consuming and laborious, and requires professional operation. It is prone to misdiagnosis and missed diagnosis when the malaria parasites are low

Pathogen gene detection (PCR) detection in malaria etiology diagnosis technology requires professional laboratory instruments and personnel

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