Preparation method and application of electrochemiluminescent polymer nanoparticles
A technology of light-emitting polymers and conductive polymers, which is applied in the fields of chemiluminescence/bioluminescence, chemical instruments and methods, and analysis through chemical reactions of materials, etc. To achieve the effects of enhanced electrochemiluminescent signal, stable retention of solution suspension uniformity, and stable structure
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Embodiment 1
[0037]Chemically link bis(2,2'-bipyridyl)(5,6-epoxy-5,6-dihydro-[1,10]phenanthroline) ruthenium to the carboxyl group of polyacrylic acid (PAA) to obtain a ruthenium complex Polymer grafted with polyacrylic acid, that is, electrochemiluminescent polymer (ECL polymer). The specific plan is as follows:
[0038] Weigh 108 mg of bis(2,2'-bipyridyl)(5,6-epoxy-5,6-dihydro-[1,10]phenanthroline) ruthenium and dissolve it in 20 mL of NaHCO 3 Aqueous solution (1mmol / L) to obtain ECL complex stock solution. Weigh 2g of PAA (molecular weight is 30000g / mol) and dissolve in 10mL of NaHCO 3 Aqueous solution (1mmol / L), get PAA stock solution. Take 3 mL of the above ECL complex stock solution and place it in a 10 mL single-neck round bottom flask, and add 1 mL of PAA stock solution. Vacuumize and fill with nitrogen, repeat three times. In an oil bath at 110° C. in the dark, under the protection of nitrogen, the reaction was carried out with magnetic stirring for 24 h. After the reaction,...
Embodiment 2
[0042] Take 1.6mL of the electrochemiluminescent polymer solution prepared in Example 1 and place it in a round bottom flask, add 0.2mL PAA (molecular weight: 30000g / mol) aqueous solution (0.4mg / mL) to it, mix well, add 0.1mLPEI (molecular weight 1800g / mol) aqueous solution (0.01mg / mL), then add 20mg of cross-linking agent trimethylolpropane-tris(3-aziridinyl)propionate, place in 60°C water bath, stir (400 rpm min) to react for 10h. Finally, the reaction solution was dialyzed in water for 24 hours to remove excess cross-linking agent to obtain electrochemiluminescent polymer nanoparticles. In this example, the electrochemiluminescent polymer and PAA undergo a cross-linking reaction, and PEI is electrostatically assembled on the ECL polymer or (and) PAA, and then cross-linked and fixed in the structure of nanoparticles, at this time PEI It is doped inside the nanoparticles by electrostatic interaction.
Embodiment 3
[0044] 1.6 mL of the electrochemiluminescent polymer solution prepared in Example 1 was placed in a round bottom flask, and 0.2 mL of an aqueous solution (0.4 mg / mL) of PAA (molecular weight: 30000 g / mol) was added. Add 1mg EDC to 0.1mL PEI (molecular weight: 1800g / mol) aqueous solution (0.01mg / mL), shake well, then add to the above-mentioned round bottom flask, EDC activates the carboxyl groups in the electrochemiluminescence polymer and PAA. Stir (400 rpm) at room temperature for 1 h to make the activated carboxyl groups react with the amino groups on PEI to undergo a cross-linking reaction. Finally, the reaction solution was dialyzed in water for 24 hours to remove excess activator, and then the electrochemiluminescent polymer nanoparticles were obtained. In this example, the electrochemiluminescence polymer, PAA and PEI all underwent a cross-linking reaction and were immobilized in nanoparticles.
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