Unlock instant, AI-driven research and patent intelligence for your innovation.

An electrochemiluminescent biosensor for detecting mmp-14 and its preparation method

A technology of MMP-14 and luminescent organisms, which is applied in the field of medical devices, can solve the problems of the influence of sensor detection sensitivity, the difficulty of applying detection of MMPs, and the lack of specificity of biosensing technology, so as to achieve the effect of reducing non-specific adsorption

Active Publication Date: 2021-10-29
NORTHWEST UNIV
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, peptide cleavage fragments and fluorescent molecules or electroactive molecule binding fragments often produce non-specific adsorption, resulting in false positive signals
Moreover, these sensors are often of the Signal OFF type due to the limitation of the detection principle. It is likely that the signal molecules fall off due to loose fixation and generate false signals. These factors will affect the detection sensitivity of the sensor.
Therefore, due to the lack of specificity of the above biosensing techniques, it is difficult to apply clinically to the detection of active site-directed MMPs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An electrochemiluminescent biosensor for detecting mmp-14 and its preparation method
  • An electrochemiluminescent biosensor for detecting mmp-14 and its preparation method
  • An electrochemiluminescent biosensor for detecting mmp-14 and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] A method for preparing an electrochemiluminescence biosensor for detecting MMP-14, the specific preparation steps are as follows:

[0039] (1) fullerene-chitosan (C 60 -Chit) preparation

[0040] First configure 0.2-2mg / mL of C 60 Solution, ultrasonic 20-30min, guarantee C 60Uniformly dispersed in the solution, and then configure a chitosan solution with a mass concentration of 0.5%. Mix the two solutions in equal volume and stir for 40-60min to ensure that C 60 Uniformly dispersed in chitosan solution, that is, C 60 -Chit.

[0041] (2) Preparation of MMP-14 protein-specific recognition probe

[0042] The polypeptide inhibitor Ap1 was dissolved in Tris-HCl (pH 8.0) buffer, and the concentration of Ap1 was 3-50 μM.

[0043] (3) Assembly of the electrochemiluminescence sensor

[0044] a. Polish the glassy carbon electrode with a diameter of 3mm with 1.0μM, 0.3μM, 0.05μM Al2O3 powder in sequence, then clean it with ethanol and deionized water in sequence, and then ...

specific Embodiment 1

[0058] A method for preparing an electrochemiluminescence biosensor for detecting MMP-14, the specific preparation steps are as follows:

[0059] (1) fullerene-chitosan (C 60 -Chit) preparation

[0060] First configure 0.2mg / mL of C 60 Solution, ultrasonic 20min, guarantee C 60 Uniformly dispersed in the solution, and then configure a chitosan solution with a mass concentration of 0.5%. Mix the two solutions in equal volumes and stir for 40 min to ensure that C 60 Uniformly dispersed in chitosan solution, that is, C 60 -Chit.

[0061] (2) Preparation of MMP-14 specific recognition signal probe

[0062] The polypeptide inhibitor Ap1 was dissolved in Tris-HCl (pH 8.0) buffer, and the concentration of Ap1 was 3 μM.

[0063] (3) Assembly of the electrochemiluminescence sensor

[0064] a. Polish the glassy carbon electrode with a diameter of 3mm with 1.0μM, 0.3μM, and 0.05μM Al2O3 powder in sequence, then ultrasonically clean it with ethanol and deionized water in turn, and...

specific Embodiment 2

[0069] A method for preparing an electrochemiluminescence biosensor for detecting MMP-14, the specific preparation steps are as follows:

[0070] (1) fullerene-chitosan (C 60 -Chit) preparation

[0071] First configure 1mg / mL of C 60 Solution, ultrasonic 25min, guarantee C 60 Uniformly dispersed in the solution, and then configure a chitosan solution with a mass concentration of 0.5%. Mix the two solutions in equal volumes and stir for 45 min to ensure that C 60 Uniformly dispersed in chitosan solution, that is, C 60 -Chit.

[0072] (2) Preparation of MMP-14 specific recognition signal probe

[0073] The polypeptide inhibitor Ap1 was dissolved in Tris-HCl (pH 8.0) buffer, and the concentration of Ap1 was 9 μM.

[0074] (3) Assembly of the electrochemiluminescence sensor

[0075] a. The glassy carbon electrode with a diameter of 3mm is polished with 1.0μM, 0.3μM, 0.05μM Al2O3 powder in turn, and then ultrasonically cleaned with ethanol and deionized water in turn, and t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to an electrochemiluminescence biosensor for detecting tumor marker MMP-14 and a preparation method thereof. The electrochemiluminescence biosensor for detecting MMP-14 obtained in the present invention, when the sensor interacts with MMP-14, MMP-14 14 binds to the electrode surface due to homodimerization with Ap1, and can bind the signal probe Ap2-Ru to the electrode surface through heterodimerization with Ap2. As the concentration of MMP-14 increases, the binding to the electrode surface As the concentration of Ap2-Ru increases, the electrochemiluminescence signal increases, and based on this, high selectivity and high sensitivity detection of MMP-14 is realized; it has the advantages of high sensitivity, high selectivity, high stability, and low cost.

Description

technical field [0001] The invention belongs to the field of medical equipment, and relates to an electrochemiluminescence biosensor for detecting MMP-14 and a preparation method thereof. Background technique [0002] Cancer remains by far the leading cause of death worldwide. Despite efforts to develop new tests and treatments, 90 percent of patients die from systemic disease caused by the spread of cancer cells. According to reports, matrix metalloproteinases (MMPs) are involved in the process of metastasis and invasion of various cancer cells, and studies have shown that the high expression of a certain MMP is positively correlated with the incidence of specific cancers, indicating that MMPs may be used as a detection of various diseases markers and therapeutic targets. Therefore, the development of sensitive and accurate MMPs sensing technology is of great significance for the clinical diagnosis and treatment of cancer. [0003] Many fluorescent or electrochemilumines...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/26G01N27/30G01N27/327G01N33/543G01N33/573G01N33/574
CPCG01N21/76G01N27/26G01N27/308G01N27/3275G01N33/5438G01N33/573G01N33/57484G01N2333/96494
Inventor 马芬孙利娜段宇宏乔新蕊
Owner NORTHWEST UNIV