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Preparation method of pyrrole/N-(2-carboxyethyl) pyrrole composite nanoparticles and application thereof

A composite nanoparticle, carboxyethyl technology, applied in the direction of chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., to achieve the effect of high specific surface area, good selectivity, and enhanced electrochemiluminescence signal

Inactive Publication Date: 2012-05-23
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, N-(2-carboxyethyl)pyrrole monomer (English name 1H-Pyrrole-1-propanoic acid) is used as functional reagent and pyrrole monomer to prepare pyrrole / N-(2-carboxy Ethyl)pyrrole composite nanoparticles and its application to the preparation of thrombin ECL probes have not been reported yet

Method used

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  • Preparation method of pyrrole/N-(2-carboxyethyl) pyrrole composite nanoparticles and application thereof
  • Preparation method of pyrrole/N-(2-carboxyethyl) pyrrole composite nanoparticles and application thereof
  • Preparation method of pyrrole/N-(2-carboxyethyl) pyrrole composite nanoparticles and application thereof

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Embodiment 1

[0031] 1. Pretreatment of pyrrole monomer

[0032] Commercially available pyrrole was distilled under reduced pressure at 0.02MPa, and the fractions at 70-80°C were collected to obtain pyrrole monomer, which was protected with nitrogen, and stored in a refrigerator at 4°C in the dark for future use.

[0033] 2. Chemical reaction

[0034] Take 0.5026g of N-(2-carboxyethyl)pyrrole monomer and 0.5mL of pyrrole monomer and add them to a conical flask filled with 1mL of n-pentanol. The molar ratio of the monomer is 1:0.5, shake and dissolve, add 60mL of sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, stir thoroughly for 30 minutes, slowly add 8mL of FeCl with a substance concentration of 1.35mol / L 3 ·6H 2 O aqueous solution, pyrrole monomer and n-amyl alcohol, sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, FeCl with a substance concentration of 1.35mol / L 3 ·6H 2 The volume ratio of the O aqueous solution was 1:2:120:16,...

Embodiment 2

[0044] In the chemical reaction step 2 of this embodiment, take 0.1256 g of N-(2-carboxyethyl) pyrrole monomer and 0.5 mL of pyrrole monomer, and add them to an Erlenmeyer flask filled with 0.75 mL of n-pentanol. The molar ratio of N-(2-carboxyethyl)pyrrole monomer to N-(2-carboxyethyl)pyrrole monomer is 1:0.125, shake to dissolve, add 40mL of sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, fully stir for 30 minutes, and slowly add 6mL of FeCl with a concentration of 1.35mol / L 3 ·6H 2 O aqueous solution, pyrrole monomer and n-amyl alcohol, sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, FeCl with a substance concentration of 1.35mol / L 3 ·6H 2 The volume ratio of the O aqueous solution was 1:1.5:80:12, and the reaction was stirred at room temperature for 12 hours to obtain a mixed solution containing pyrrole / N-(2-carboxyethyl)pyrrole composite nanoparticles. Other steps are identical with embodiment 1. Prepare pyrrol...

Embodiment 3

[0046] In the chemical reaction step 2 of this embodiment, take 1.0052 g of N-(2-carboxyethyl) pyrrole monomer and 0.5 mL of pyrrole monomer, and add them to an Erlenmeyer flask filled with 1.35 mL of n-pentanol. The molar ratio of N-(2-carboxyethyl)pyrrole monomer to N-(2-carboxyethyl)pyrrole monomer is 1:1, shake to dissolve, add 80mL of sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, fully stir for 30 minutes, and slowly add The amount concentration of 10.55mL substance is FeCl of 1.35mol / L 3 ·6H 2 O aqueous solution, pyrrole monomer and n-amyl alcohol, sodium lauryl sulfate aqueous solution with a mass concentration of 86.8g / L, FeCl with a substance concentration of 1.35mol / L 3 ·6H 2 The volume ratio of the O aqueous solution was 1:2.7:160:21.1, and the reaction was stirred at room temperature for 12 hours to obtain a mixed solution containing pyrrole / N-(2-carboxyethyl)pyrrole composite nanoparticles. Other steps are identical with embodimen...

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Abstract

The invention relates to a preparation method of pyrrole / N-(2-carboxyethyl) pyrrole composite nanoparticles, comprising the following steps of: pretreating pyrrole monomer, chemically reacting, separating and drying. The preparation method has the advantages of simple and convenient operation as well as nano-grade and epigranular prepared product. The prepared pyrrole / N-(2-carboxyethyl) pyrrole composite nanoparticles have higher specific surface area and more carboxyl functional groups, increase the load capacity of amino ruthenium bipyridine derivatives and ensure that electrochemical luminescence signals are enhanced. A thrombin electrochemical luminescence probe prepared from the pyrrole / N-(2-carboxyethyl) pyrrole composite nanoparticles can be used for detecting thrombin, has favorable selectivity, sensitivity and detection limit and can be used on a medical trace detection instrument.

Description

technical field [0001] The invention belongs to the technical field of heterocyclic compounds containing two or more heterocyclic rings, with nitrogen atoms as the only ring heteroatoms, in particular to a pyrrole / N-( 2-carboxyethyl)pyrrole composite nanoparticles. Background technique [0002] Thrombin is an important physiological protease, which plays an important role in physiological and pathological processes such as blood coagulation, inflammation and wound healing. Because patients with malignant tumors often have different degrees of bleeding tendencies, the invasion of tumor tissue to surrounding tissues, the formation of metastasis, and the changes in tumor tissue and the blood of tumor patients can all lead to changes in the patient's blood coagulation mechanism, and the concentration and activity of thrombin As an important index to measure the coagulation mechanism, it is of great significance to reveal the mechanism of tumorigenesis, or as an early diagnosis,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G73/06G01N21/76
Inventor 张成孝贾丽娟漆红兰孙波
Owner SHAANXI NORMAL UNIV