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A compound bacterial agent for promoting the rapid decomposition of agricultural waste and its preparation method

A technology of agricultural waste and composite bacterial agent, which is applied in the field of composite bacterial agent that promotes the rapid decomposition of agricultural waste and its production, can solve the problems of slow degradation rate of straw and poor cellulose degradation efficiency, and achieve less expenditure, High safety and high degradation efficiency

Active Publication Date: 2020-11-24
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a compound bacterial agent for promoting the rapid decomposition of agricultural waste and its preparation method, which solves the problems that the existing EM compound bacterial agent degrades straw at a slow rate and the degradation efficiency of cellulose in straw is not good.

Method used

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  • A compound bacterial agent for promoting the rapid decomposition of agricultural waste and its preparation method
  • A compound bacterial agent for promoting the rapid decomposition of agricultural waste and its preparation method
  • A compound bacterial agent for promoting the rapid decomposition of agricultural waste and its preparation method

Examples

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Effect test

Embodiment 1

[0045] A composite bacterial agent for promoting rapid decomposition of agricultural waste, prepared from the following raw materials in parts by weight: 50 parts of Aspergillus oryzae culture, 15 parts of yeast culture, 15 parts of Aspergillus niger culture, and Bacillus subtilis culture 15 parts and 1 part of lactic acid bacteria culture. Concretely prepare according to the following steps:

[0046] Step 1, prepare Aspergillus oryzae culture

[0047] Step 1.1, activating the Aspergillus oryzae strain, and eluting the Aspergillus oryzae spores with sterile water to obtain the Aspergillus oryzae spore suspension;

[0048] Step 1.2: Soak soybeans in water for 4 hours, drain the water, steam them until cooked, and cool to 30°C to obtain cooked soybeans; grind wheat straw into powder and pass through a 60-mesh sieve to obtain wheat straw powder; then mix cooked soybeans and wheat The straw powder is uniformly mixed according to the mass ratio of 1:2 to obtain the Aspergillus or...

Embodiment 2

[0066] A composite microbial agent for promoting rapid decomposition of agricultural waste, prepared from the following raw materials in parts by weight: 40 parts of Aspergillus oryzae culture, 20 parts of yeast culture, 20 parts of Aspergillus niger culture, and Bacillus subtilis culture 10 parts and 2 parts of lactic acid bacteria culture. The Aspergillus oryzae is Aspergillus oryzae Huyao 3.042; the yeast is Saccharomyces cerevisiae; the Aspergillus niger is Aspergillus niger AS3.316; the Bacillus subtilis is Bacillus subtilis ACCCNo.03189; the lactic acid bacteria are salt-tolerant lactic acid bacteria sCGMCC 3792.

[0067] Concretely prepare according to the following steps:

[0068] Step 1, prepare Aspergillus oryzae culture

[0069] Step 1.1, activating the Aspergillus oryzae strain, and eluting the Aspergillus oryzae spores with sterile water to obtain the Aspergillus oryzae spore suspension;

[0070] Step 1.2: Soak soybeans in water for 6 hours, drain the water, st...

Embodiment 3

[0088] A compound bacterial agent for promoting rapid decomposition of agricultural waste, prepared from the following raw materials in parts by weight: 60 parts of Aspergillus oryzae culture, 10 parts of yeast culture, 10 parts of Aspergillus niger culture, and Bacillus subtilis culture 20 parts and 1.5 parts of lactic acid bacteria culture. The Aspergillus oryzae is Aspergillus oryzae Huyao 1.08; the yeast is Saccharomyces cerevisiae; the Aspergillus niger is Aspergillus niger AS3.316; the Bacillus subtilis is Bacillus subtilis ACCCNo.03189; the lactic acid bacteria are bifidobacteria , the specific preparation steps are the same as in Example 1.

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Abstract

The invention discloses a compound bacterial agent for promoting rapid decomposition of agricultural waste. The compound bacterial agent is prepared from the following raw materials in parts by weight: 40-60 parts of an Aspergillus oryzae culture, 10-20 parts of a saccharomycete culture, 10-20 parts of an Aspergillus niger culture, 10-20 parts of a bacillus subtilis culture and 1-2 parts of a lactic acid bacteria culture. Aspergillus oryzae, saccharomycete, Aspergillus niger, bacillus subtilis and lactic acid bacteria adopted in the compound bacterial agent are high in safety, do not generate toxic side effect and can be generate a synergistic effect, so that the degradation of agricultural waste such as crop straws, the degradation rate is high, and the degradation efficiency of cellulose in the straws is high. Besides, the invention further provides a preparation method of the compound bacterial agent for promoting rapid decomposition of the agricultural waste.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of agricultural waste, and in particular relates to a composite bacterial agent for promoting rapid decomposition of agricultural waste and a preparation method thereof. Background technique [0002] my country is a large agricultural production country, which produces a large amount of agricultural waste every year, especially crop straw, including corn straw, wheat straw, sorghum straw, rice straw, animal manure, etc. These straws contain a lot of carbohydrates, proteins and fats. If the agricultural wastes mainly of crop stalks are not utilized and discarded, a large amount of resources will be wasted. [0003] In the prior art, crop stalks and other wastes are mainly used as the main raw materials for livestock feed, and there is little research on their decomposition in returning to the field, and even less research on their decomposition technology. In the field of biology, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/18C05F11/08C12R1/69C12R1/865C12R1/685C12R1/125C12R1/01
CPCC05F11/08C12N1/14C12N1/18C12N1/20
Inventor 李继光孙慧
Owner BEIBU GULF UNIV