Molecular target cell-free DNA for evaluating curative effects of immune drugs for cancer and application thereof
A molecular target, cancer immunity technology, applied in the field of medical biological detection, to achieve great clinical application value and the effect of solving technical problems
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Embodiment 1
[0025] Example 1 Efficacy evaluation of atezolizumab
[0026] 50 patients with advanced bladder cancer (TNM stage IV) were collected from the hospital, including 36 males and 14 females, of which 42 were positive for PD-L1 expression and 8 were negative. Give atezolizumab (atezolizumab, Genentech, USA) 1200mg every 3 weeks, an intravenous infusion lasts 60 minutes, and needs to be diluted before intravenous infusion.
[0027] Blood samples were drawn from patients before administration and 2 days after the first administration, and then these blood samples were put into centrifuge tubes containing 5 ml of EDTA, centrifuged at 3000G at room temperature for 10 minutes with a centrifuge, and the supernatant was extracted. QIAamp DNA Kit (QIAGEN USA) was used to purify cell-free DNA according to the manufacturer's procedure. Partially purified DNA with Quant-iT TM dsDNAAssay Kit (ThermoFisher, USA) quantified its concentration.
[0028] Two days after the administration, the ...
Embodiment 2
[0040] Example 2 Nivolumab (nivolumab) curative effect evaluation
[0041] 50 patients with metastatic squamous non-small cell lung cancer were collected from the hospital, including 43 males and 7 females, of which 39 were positive for PD-L1 expression and 11 were negative. Nivolumab was administered intravenously at 3 mg / kg every 2 weeks.
[0042] Blood samples were drawn from the patients before administration and 2 days after the first administration, and then these blood samples were put into centrifuge tubes containing 5 ml of EDTA, centrifuged at 3000G for 10 minutes at room temperature, and the supernatant was extracted. QIAamp DNA Kit (QIAGEN USA) was used to purify cell-free DNA according to the manufacturer's procedure. Partially purified DNA with Quant-iT TM dsDNAAssay Kit (ThermoFisher, USA) quantified its concentration.
[0043] After 2 days of administration, the patients whose cell-free DNA content in the blood sample doubled were divided into the onset gr...
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