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Preparation method of cyclodextrin inclusion multivalent specific swine transfer factor

A technology of transfer factor and cyclodextrin, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations with non-active ingredients, etc., can solve the problems of low active ingredients and easy to be destroyed by gastric acid, so as to achieve the original properties unchanged and not easy to Stomach acid destruction, antiviral effect outstanding effect

Inactive Publication Date: 2017-08-04
SHIJIAZHUANG SHIMU ANIMAL HUSBANDRY PHARMA CO LTD
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  • Abstract
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Problems solved by technology

[0002] Viral diseases of livestock and poultry seriously threaten the development of the majority of farmers and even the entire national economy. The loss caused by virus infection of livestock and poultry in my country can reach tens of billions of yuan every year. Improving animal immunity is a major way to solve animal virus infection , among the drugs for enhancing the immunity of animals, transfer factor has withstood a long-term test and is one of the widely representative drugs. At present, the existing transfer factor has low active ingredients and is easily destroyed by gastric acid when taken orally.

Method used

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  • Preparation method of cyclodextrin inclusion multivalent specific swine transfer factor
  • Preparation method of cyclodextrin inclusion multivalent specific swine transfer factor
  • Preparation method of cyclodextrin inclusion multivalent specific swine transfer factor

Examples

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preparation example Construction

[0030] A method for preparing cyclodextrin inclusion polyvalent specific porcine transfer factor proposed by the present invention comprises the following steps:

[0031] (1) Preparation of raw materials: select healthy pigs with no medical history and immunoenhancing treatment to slaughter, take spleens on ice, and freeze them;

[0032] (2) Pretreatment: After weighing the pig spleen, wash the pig spleen with cold three-distilled water, cut off its fascia and adipose tissue, and then wash it with cold three-distilled water;

[0033] (3) Fragmentation: the cleaned pig spleen is shredded, added with cold physiological saline, and crushed with a high-speed tissue masher to obtain a homogenate;

[0034] (4) Freezing and thawing: the homogenate is placed in the freezer and then thawed, and several times of alternate freezing and thawing are carried out;

[0035] (5) Extraction: add cold physiological saline, place in a refrigerated centrifuge for centrifugation, take the upper bl...

Embodiment 1

[0047] Embodiment one: a kind of preparation method of cyclodextrin inclusion polyvalent specific porcine transfer factor, comprises the following steps:

[0048] (1) Preparation of raw materials: select healthy 6-month-old pigs with no medical history for immunoenhancement treatment, slaughter them, take their spleens on ice, and transfer them to store at -20°C;

[0049] (2) Pretreatment: After weighing the pig spleen, wash the pig spleen with cold three-distilled water, cut off its fascia and adipose tissue with scissors, and then wash it with cold three-distilled water;

[0050](3) Broken: Shred the cleaned pig spleen, add 2 times the volume of cold normal saline, mash 3 times with a high-speed tissue masher at 1000 rpm, each time for 3 minutes, to obtain a homogenate;

[0051] (4) Freezing and thawing: the specific freezing temperature is -80°C, the specific melting method and temperature are 30°C in a water bath, and the specific number of alternate freezing and thawing t...

Embodiment 2

[0057] Embodiment 2: A preparation method of cyclodextrin inclusion polyvalent specificity porcine transfer factor is as follows: in step (1) of embodiment 1, specific immune enhancement treatment is given to the spleen donor pig before spleen extraction, and the piglets are immunized with a A group of polypeptide combined antigens containing several types of antigenic epitopes of pathogenic microorganisms in livestock and poultry, so that the prepared transfer factor can not only enhance the function of non-specific immunity but also have the function of improving specific anti-pathogenic microorganisms, specifically to immunize piglets with several epidemic Antigens of diseases and common diseases, so that the prepared transfer factor has the function of immunizing epidemic diseases and common diseases, and other steps are the same as in Example 1.

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Abstract

The invention belongs to the technical field of transfer factor preparation, and provides a preparation method of a cyclodextrin inclusion multivalent specific swine transfer factor. The preparation method comprises the following steps: preparing raw materials, carrying out pretreatment, crushing, freezing and thawing, extracting, carrying out ultrafiltration, sterilizing, carrying out inclusion, sub-packaging, and freeze-drying to finally obtain transfer factor powder for the inclusion of animals. The innovation point of the preparation method is that on the one hand, special treatment is carried out on the raw materials before preparation, namely, specific immunological enhancement treatment is carried out on a pig for supplying the spleen before preparation, and a piglet is immunized with a group of polypeptide combined antigens containing a number of types of livestock and poultry pathogenic microbial epitopes, so that the prepared transfer factor not only has the function of enhancing non-specific immunity, but also has the function of improving the specific anti-pathogenic microorganisms; on the other hand, a cyclodextrin inclusion technology solves the technical problems that the existing transfer factor is easy to damage by gastric acid when being orally taken, and is low in utilization rate; therefore, the cyclodextrin inclusion multivalent specific swine transfer factor solves the technical problems which are not solved for a long time in the prior art.

Description

technical field [0001] The invention belongs to the technical field of transfer factor preparation, and relates to a method for preparing cyclodextrin inclusion polyvalent specific porcine transfer factor. Background technique [0002] Viral diseases of livestock and poultry seriously threaten the development of the majority of farmers and even the entire national economy. The annual loss caused by virus infection of livestock and poultry in my country can reach tens of billions of yuan. Improving animal immunity is a main way to solve animal virus infection , among the drugs for enhancing the immunity of animals, transfer factor has withstood a long-term test and is one of the widely representative drugs. At present, the existing transfer factor has low active ingredients and is easily destroyed by gastric acid when taken orally. . Contents of the invention [0003] The present invention proposes a method for preparing cyclodextrin inclusion polyvalent specific porcine tra...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K38/19A61P31/00
CPCA61K38/19
Inventor 陈巨清
Owner SHIJIAZHUANG SHIMU ANIMAL HUSBANDRY PHARMA CO LTD
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