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Application of Synsepalum dulcificum leaf extract in preparation of molecular targeting drugs

A technology of molecular targeted drugs and miracle fruit leaves, which is applied in the direction of drug combination, plant/algae/fungus/moss components, anti-tumor drugs, etc., can solve problems not related to anti-tumor, and achieve obvious effects and small side effects Effect

Inactive Publication Date: 2017-08-11
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of anti-tumor, there is only anti-tumor research in vitro, and it does not involve in-vivo anti-tumor research and related application development.

Method used

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  • Application of Synsepalum dulcificum leaf extract in preparation of molecular targeting drugs
  • Application of Synsepalum dulcificum leaf extract in preparation of molecular targeting drugs
  • Application of Synsepalum dulcificum leaf extract in preparation of molecular targeting drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of miraculous fruit leaf extract

[0031] Weigh 400g of miracle fruit leaves, after the homogenate is broken, add 80% (v / v) ethanol aqueous solution, the solvent volume ml is 15 times of the solid mass g, and carry out ultrasonic extraction, the extraction temperature is 50 ℃. Extract 3 times according to the above method, and combine the extracts. The above alcohol extract was distilled under reduced pressure at 40°C, and freeze-dried at minus 35°C for 24 hours to obtain the crude extract of miracle fruit leaves.

[0032] The above-mentioned crude extract of miraculous fruit leaves was dissolved in water, and treated with macroporous resin AB-8, first passed through a column with distilled water to remove sugar, and then eluted with 60% (v / v) ethanol, and the eluate was collected. The eluate was vacuum freeze-dried at a temperature of -50° C. for 48 hours to obtain a solid miraculous leaf extract powder.

[0033] Component Analysis of Mi...

Embodiment 2

[0046] Example 2: In vivo anti-tumor angiogenesis experiment of miraculous fruit leaf extract in zebrafish

[0047] 1. Zebrafish acute toxicity test in vivo

[0048] Zebrafish: fli1a-EGFP transgenic zebrafish was purchased from Shanghai Model Biotechnology Co., Ltd.

[0049] Experimental materials: Miracle fruit leaf extract prepared in Example 1; 0.1% DMSO, Shanghai Model Biotechnology Co., Ltd.

[0050] experimental method:

[0051] (1) Miracle fruit leaf extracts of different concentrations were dissolved in 0.1% DMSO (the concentrations of the miracle fruit leaf extracts were set as: 0.1, 1, 2.5, 5, 10, 25, 50, 100, 250, 500, 1000 μg / mL ),spare.

[0052] (2) Collect the newly hatched zebrafish embryos and place them in a petri dish, place them in an incubator at 28.5°C for culture, and when they reach 24-72hpf (hours after incubation), add the embryos in the petri dish to a 24-well plate, 20 tails of wells were added with samples at the same time, and placed in an incu...

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Abstract

The invention relates to application of Synsepalum dulcificum leaf extract in the preparation of molecular targeting drugs; the Synsepalum dulcificum leaf extract is extracted via the steps of homogenizing and crushing Synsepalum dulcificum leaf into Synsepalum dulcificum leaf powder, ultrasonically extracting with 70-90% (v / v) ethanol solution, and centrifugally collecting supernate; distilling the supernate under reduced pressure, and lyophilizing to obtain Synsepalum dulcificum leaf crude extract; dissolving the Synsepalum dulcificum leaf crude extract in water, treating with macroporous resin, eluting with an eluent, and collecting eluant; distilling the eluant under reduced pressure, and lyophilizing to obtain Synsepalum dulcificum leaf extract powder. Molecular targeting drugs containing the Synsepalum dulcificum leaf extract are small toxic and side effects, have significant effect in resisting tumor angiogenesis and are good in safety and reliability.

Description

technical field [0001] The present invention relates to the application of the miraculous fruit leaf extract, in particular to the application of the miraculous fruit leaf extract in the preparation of molecular targeted medicines. Background technique [0002] Malignant tumor is one of the most common and serious diseases that directly threaten human life in the world today. In recent years, with the continuous development of molecular oncology and molecular pharmacology and the gradual elucidation of the nature of tumors, the wide application of advanced technologies such as drugs, genetic engineering, combinatorial chemistry, and large-scale rapid screening has accelerated the development of new anti-tumor drugs. According to the traditional classification and research progress of antineoplastic drugs, antineoplastic drugs are divided into cytotoxic drugs; drugs affecting hormone balance; other antineoplastic drugs (including biological response modifiers and new molecula...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00
CPCA61K36/185A61K2236/333A61K2236/55
Inventor 马飞跃付琼张秀梅刘玉革
Owner SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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