Partial base deletion muscle inhibin gene capable of being expressed in mouse and application

Abstract

The invention discloses a partial base deletion muscle inhibin gene capable of being expressed in a mouse and application. An MSTN gene comprises two introns and three exon subsequence. 6 bases are deleted at the 175th-180th bit of the third exon are deleted, a cutting carrier containing partial base deletion muscle inhibin genes is constructed, a knock-out mouse containing the partial base deletion muscle inhibin genes is prepared, the cutting carrier containing the partial base deletion muscle inhibin genes is applied to MSTN organism character researches, the muscle amount of mouse skeletal muscle is increased, a double muscling phenomenon is generated, and the mouse basal metabolic rate and mouse body temperature are reduced. Through a genetic engineering method, base knock-out is performed on the original MSTN gene of the mouse, normal physiological functions of the organism are not affected while the muscle amount of the mouse skeletal muscle is increased, the muscle amount of the mouse can be quickly improved, and the partial base deletion muscle inhibin gene has large application prospect.

Description

technical field [0001] The invention belongs to the genetic engineering technology in the field of biotechnology, and specifically relates to a partial base deletion myostatin gene which can be expressed in mice and its application. Background technique [0002] MSTN is a myostatin gene that belongs to the TGF-β superfamily. Its synthesis is mainly completed by skeletal muscle and belongs to the secretory type of polypeptide. As a member of the TGF-β superfamily, it has the same biological properties as this family. Structure: including the secretory signal peptide at the N-terminus, the proteolytic processing site (RSRR) and the mature peptide region at the C-terminus, containing cysteine ​​(cystine knot) structure, these components form a 52kDa The inactive precursor protein can only form a 26kDa active peptide after being processed by the intermediate proteolysis site, so as to exert its biological function. The composition of MSTN gene cDNA: the nucleotide sequence of 1...

AI Technical Summary

Problems solved by technology

However, there are very few naturally mutated MSTNs in animals, so how to base-knock out the original MSTN gene in mice so that it can play a role in mammals is an urgent technical problem in this field. There are many challenges in the process: firstly, how to obtain the gene sequence that can be expressed, and secondly, to construct the targeting vector. Considering the targeting efficiency of the targeting vector, the stability of the foreign gene mRNA, and the influence of the secondary structure of the mRNA on the translation efficiency, While optimizing, be sure to avoid the formation of secondary structures that hinder the expression of specific mRNAs

The above challenges hinder the optimization of gene sequences

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