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A strain of Paleobacterium hominis fx02 and its application in denitrification of wastewater

A technology of Paleobacterium hominis and strains, applied in the field of microbial denitrification, can solve the problems of low efficiency, limited application and development, discontinuous efficiency of denitrification process, etc., and achieve the effect of good application prospects

Active Publication Date: 2020-03-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification technology is that the two processes of nitrification and denitrification are independent of each other, resulting in discontinuity and low efficiency of the denitrification process, which greatly limits the application and development of traditional biological denitrification technology

Method used

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  • A strain of Paleobacterium hominis fx02 and its application in denitrification of wastewater
  • A strain of Paleobacterium hominis fx02 and its application in denitrification of wastewater
  • A strain of Paleobacterium hominis fx02 and its application in denitrification of wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The screening of heterotrophic nitrification-aerobic denitrification bacterial strain, concrete steps are as follows:

[0028] 1. Acclimatization and enrichment: The experimental sludge comes from the activated sludge in the aerobic section of Guangzhou Datansha Wastewater Treatment Plant. Take 10 g of fully mixed activated sludge and place them in 500 mL of nitrification culture solution and denitrification culture solution respectively. Place in a conical flask in a shaker, set the temperature at 30°C, rotate at 150 r / min, and acclimatize with 3 days as a cycle. After each cycle, the culture medium in the bottle was left to stand for a while, the supernatant was poured off, and 200 mL of fresh culture medium was added again for acclimatization. During this period, the removal of ammonia nitrogen and nitrate nitrogen was detected.

[0029] 2. Separation and purification: gradiently dilute 1mL of acclimatization solution to 10 with sterile water -1 、10 -3 、10 -5 10...

Embodiment 2

[0035] Growth and denitrification characteristics of the FX02 bacterial strain described in embodiment 1

[0036] 1. After expanding the culture of the FX02 strain, resuspend the bacteria with sterile water (to a volume of 1g / L), and then inoculate 10% of the inoculum into a 250mL Erlenmeyer flask containing 100mL of heterotrophic nitrification medium, fully Shake well, incubate on a shaker at 30°C and 120r / min for 72 hours, take samples regularly to measure NH in the supernatant 4 + -N, NH 2 OH, NO 3 - -N, NO 2 - -N, COD, TN and OD 600 value.

[0037] see results figure 2 ,according to figure 2 The results showed that when the FX02 strain used ammonia nitrogen as the only nitrogen source, the FX02 strain entered the logarithmic phase after 12 hours of growth, and the strain entered the decay phase after a short stable phase. While the strain was growing, NH 4 + -N, TN and COD decreased, NH 4 + -N was completely removed at 72 h, the maximum NH 4 + The removal...

Embodiment 3

[0042] Example 3 The application of the FX02 strain described in Example 1 to enhance denitrification.

[0043] water quality index NH 4 + -N

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Abstract

The invention belongs to the technical field of microbial denitrification, and specifically discloses a strain of Pallidus human pallidus FX02 and its application in wastewater denitrification. The strain was deposited in the Guangdong Microbial Strain Collection Center (GDMCC) on August 17, 2016, and the preservation number is GDMCC No: 60062. The strain is a heterotrophic nitrifying-aerobic denitrifying bacterium, which makes it possible to carry out nitrification and denitrification simultaneously, and has great application value in sewage treatment.

Description

technical field [0001] The invention relates to the technical field of microbial denitrification, and in particular relates to a strain of Pallidum human FX02 and its application in waste water denitrification. Background technique [0002] In view of the deteriorating quality of water environment and the increasingly serious phenomenon of water eutrophication, efficient and rapid removal of nitrogen in water is an important topic in the field of water pollution prevention and control. The traditional biological denitrification technology is that the two processes of nitrification and denitrification are independent of each other, which leads to the discontinuity and low efficiency of the denitrification process, which greatly limits the application and development of the traditional biological denitrification technology. At present, the development and application of some new biological denitrification technologies provide new ideas for biological denitrification, and many ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 曹刚李紫惠黄郑郑陈海升莫测辉
Owner JINAN UNIVERSITY