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Quality control method and fingerprint of spleen invigorating and kidney benefiting prescription

A quality control method, a technology of invigorating the spleen and kidney, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex components of traditional Chinese medicine and unclear active ingredients.

Inactive Publication Date: 2017-08-18
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The internal quality assurance of traditional Chinese medicine and its compound is also the basic requirement of the modernization of traditional Chinese medicine. The composition of traditional Chinese medicine compound is complex, and most of the active ingredients are not clear. It is urgent to establish internal quality evaluation standards for traditional Chinese medicine.

Method used

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  • Quality control method and fingerprint of spleen invigorating and kidney benefiting prescription
  • Quality control method and fingerprint of spleen invigorating and kidney benefiting prescription
  • Quality control method and fingerprint of spleen invigorating and kidney benefiting prescription

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] A quality control method for invigorating the spleen and benefiting the kidney, comprising the following steps:

[0081] S1. Prepare the test solution of Bupi Yishen Fang;

[0082] S2. draw need testing solution and inject liquid chromatograph, record chromatogram;

[0083] S3. Import the chromatogram obtained in step S2 into the Chinese medicine chromatographic fingerprint similarity evaluation system; select the chromatographic peaks that exist in the chromatograms of different batches of Bupi Yishen formula as common peaks; use the average value calculation method to generate Bupi Yi The standard fingerprint of Shenfang; calculate the relative retention time and relative peak area of ​​each common peak;

[0084] S4. Establish the fingerprint of the Bupi Yishen formula to be detected; then compare it with the standard fingerprint described in step S3, and finally determine whether the quality of the Bupi Yishen formula to be tested is qualified;

[0085] The need te...

Embodiment 2

[0143] Further, after the liquid chromatograph described in embodiment 1 step S2, a mass spectrometer is also connected to detect the molecular weight of the common peak; the mass spectrometer adopts an electrospray ion source (ESI), and the sheath gas is 45 units , the auxiliary gas is 6 units, the spray voltage is -2.5~-3.5kV, the capillary temperature is 300~375°C, the sample is first scanned with high resolution, the second mass spectrometer is scanned with dynamic data dependence, and the collision energy is set to 20~ 45%.

[0144] Further, on the basis of Example 1, calycosin-7-O-β-D-glucoside, formononetin, calycosin, stilbene glycoside, rosmarinic acid, shikonic acid, salvianolic acid A, salvianolic acid B, astragaloside, astragaloside, astragaloside, astragaloside II, salvianolic acid B, astragaloside, codonopside, formononetin, astragaloside II, etc., accurately weighed the appropriate amount, dissolved in 20% acetonitrile, prepared Standard solution, HPLC sample i...

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Abstract

The present invention discloses a quality control method and a fingerprint of a spleen invigorating and kidney benefiting prescription. The quality control method comprises: S1, preparing a spleen invigorating and kidney benefiting prescription sample solution; S2, sucking the sample solution, injecting into a liquid chromatograph, and recording a chromatogram; S3, importing the chromatogram obtained in the step S2 into a traditional Chinese medicine chromatogram fingerprint similarity evaluation system, selecting chromatographic peaks existing in the chromatograms of different batches of the spleen invigorating and kidney benefiting prescriptions as common peaks, generating a standard fingerprint of the spleen invigorating and kidney benefiting prescription by using a mean value calculation method, and calculating the relative retention times and the relative peak areas of each common peak; and S4, establishing the fingerprint of the spleen invigorating and kidney benefiting prescription to be detected, comparing to the standard fingerprint of the spleen invigorating and kidney benefiting prescription, and judging whether the spleen invigorating and kidney benefiting prescription to be detected is qualified. With the quality control method of the present invention, the onesidedness that the quality of the spleen invigorating and kidney benefiting prescription is judged by only determining one or two chemical components is avoided, and the complete control of the quality of the compound traditional Chinese medicine is easily achieved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a quality control method and a fingerprint of a prescription for invigorating the spleen and benefiting the kidney. Background technique [0002] Chronic kidney disease (CKD) refers to kidney damage or glomerular filtration rate (GFR) <60mL / (min·1.73m 2 ) lasted for 3 months or more; renal impairment was defined as abnormal renal pathology or abnormal blood, urine, and imaging examinations. CKD is a general term for various primary and secondary kidney diseases. It is a group of chronic non-infectious diseases with progressive development. The prevalence rate in my country is increasing year by year, and it has become a serious challenge that countries all over the world must face. The entire process of CKD from normal renal function to renal failure is divided into five stages, which are equivalent to two stages of chronic nephritis and chronic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 丘小惠刘旭生张靖徐文毛炜黄志海徐鹏吴俊标
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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