Method for improving detection sensitivity of SERS substrate by using molecular template enhanced reagent

A technology of detection sensitivity and molecular template, which is applied in the detection field to achieve the effect of improving the enhancement effect, realizing ultra-trace detection, and realizing the effect of sensitivity

Inactive Publication Date: 2017-08-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the surface selection rule has been theoretically explained and confirmed, the method that can adjust the orientation of the molecules to be measured according to the surface selection rule has not been reported in reality.

Method used

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  • Method for improving detection sensitivity of SERS substrate by using molecular template enhanced reagent
  • Method for improving detection sensitivity of SERS substrate by using molecular template enhanced reagent
  • Method for improving detection sensitivity of SERS substrate by using molecular template enhanced reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Application of the SERS substrate binding enhancement reagent technology with silver nanoparticles assembled on the surface in the detection of p-mercaptoaniline (PATP) molecules

[0035] 1. Preparation of silver sol: Dissolve 36mg of silver nitrate in 200mL of water, heat rapidly to boiling, add 4mL of sodium citrate aqueous solution with a mass concentration of 1% rapidly under vigorous stirring, keep the slightly boiling state and continue to react for 1.5 hours, then naturally After cooling and stirring to room temperature, the resulting sol was brown-gray. A silver nanoparticle sol with a particle size of 70 nm was obtained. Assembly of silver nanoparticles on a glass substrate: the glass substrate was ultrasonically cleaned in water, ethanol, acetone, chloroform, acetone, ethanol, and water for 5 minutes, and then placed in 30% H 2 o 2 Aqueous solution and 98% H 2 SO 4 Water solution (volume ratio: 3:7) boiled in the mixture for 20 minutes, washed w...

Embodiment 2

[0039] Example 2: Application of Gold Nanoparticles as SERS Substrate Binding Enhancement Reagent Technology in Detection of p-Mercaptobenzoic Acid (MBA) Molecules

[0040] 1. Preparation of gold nanoparticle sol: first prepare chloroauric acid into an aqueous solution with a mass concentration of 0.01%, take 100 mL and heat it to boiling. Accurately add 2.5 mL of sodium citrate aqueous solution with a mass concentration of 1% under stirring, and continue to boil for 15 minutes. At this time, it can be observed that the solution changes from light yellow to gray quickly, then to black, and then gradually stabilizes to red. After cooling to room temperature, the gold nanoparticle sol was obtained.

[0041] 2. Assembly of gold nanoparticles on a glass substrate: ultrasonically clean the glass substrate used in the experiment in water, ethanol, acetone, chloroform, acetone, ethanol, and water for 5 minutes, and then place it in 30% H 2 o 2 Aqueous solution and concentrated H w...

Embodiment 3

[0045] Example 3: Application of silver nanoparticles as SERS substrate binding enhancement reagent technology in detecting rhodamine 6G (R6G) molecules

[0046] 1. Preparation of silver sol: Dissolve 36mg of silver nitrate in 200mL of water, heat rapidly to boiling, add 4mL of sodium citrate aqueous solution with a mass concentration of 1% rapidly under vigorous stirring, keep the slightly boiling state and continue to react for 1.5 hours, then naturally Cool and stir to room temperature. The obtained sol was brownish gray, and a silver nanoparticle sol with a particle size of 70 nm was obtained.

[0047] 2. Assembly of silver nanoparticles on a glass substrate: ultrasonically clean the glass substrate used in the experiment in water, ethanol, acetone, chloroform, acetone, ethanol, and water for 5 minutes, and then place it in 30% H 2 o 2 Aqueous solution and concentrated H with a mass concentration of 98% 2 SO 4 Aqueous solution (volume ratio is 3:7) in the mixture liqui...

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Abstract

The invention provides a method for improving detection sensitivity of a SERS substrate by using a molecular template enhanced reagent, and belongs to the detection technology field. Ultra trace detection of molecules to be measured on traditional surface enhanced raman spectroscopy (SERS) substrates is increased by 2 orders of magnitude by the method, detection sensitivity of SERS is greatly improved, and prospect prospects are wide. The SERS substrate with gold or silver nanoparticles on the surface by self assembly or vapor plating is placed in an ethanol solution of probe molecules, and immersion is carried out for 20-30 hours, and the substrate is taken out; an ethanol solution of 1-hexanethiol whose concentration is 10<-4>-10<-6>M is prepared, the substrate is immersed in ethanol solution of 1-hexanethiol for 4-10 hours, ethanol is used for cleaning, drying is carried out with nitrogen, and the SERS substrate with constructed molecular templates is obtained; SERS test results show that the molecular template enhanced reagent can effectively improve detection sensitivity of the SERS substrate. The method further provides experimental evidence for researching mechanism of SERS, and at the same time, establishes a base for developing the SERS into a representation tool of material surface special properties.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for improving the detection sensitivity of a SERS substrate by using a molecular template enhancing reagent, which realizes the ultra-trace detection of molecules to be tested on a traditional surface-enhanced Raman spectroscopy (SERS) substrate by two orders of magnitude , so that the detection sensitivity of SERS is greatly improved, and has a wide application prospect. Background technique [0002] In 1928, Indian physicist C.V.Raman first discovered the Raman scattering effect and won the 1930 Nobel Prize in Physics. The frequency, intensity, and polarization of the Raman spectrum mark the fingerprint information of the substance, from which information on the structure and composition of the substance can be obtained, so Raman spectroscopy has been widely used. Raman spectroscopy is an important means to study molecular structure, but with the practic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 阮伟东孙成彬周铁莉王蕴馨丛茜陈廷坤李亚丽李倩文王旭赵冰
Owner JILIN UNIV
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