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Application of OsNPF5.16 gene for increasing single-plant yield of paddy rice

A gene and rice technology, which is applied in the application field of OsNPF5.16 gene in improving the yield per plant of rice, can solve the problems of what type of growth and development of plants is affected, and the growth and development of plants is affected, so as to achieve the effect of increasing yield

Active Publication Date: 2017-08-29
WUHAN INST OF BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although it is known that nitrogen nutrition can promote plant growth and development, there is no systematic understanding of what type of plant growth and development nitrogen nutrition affects.
In addition, the rice NPF family has more than 80 members. It is almost unknown which member of the NPF gene family responds to nitrogen nutrition, and at what site mediates nitrogen nutrition transport, thus affecting the growth and development of the plant.

Method used

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  • Application of OsNPF5.16 gene for increasing single-plant yield of paddy rice
  • Application of OsNPF5.16 gene for increasing single-plant yield of paddy rice
  • Application of OsNPF5.16 gene for increasing single-plant yield of paddy rice

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 OsNPF5.16 Construction of Gene Overexpression Plants

[0024] Extract the RNA of rice Zhonghua 11 and reverse transcribe it into cDNA, using the primer pair:

[0025] F1: 5'- GGTACC ATGGACGCGGGGGCGGAGCCCCTC-3'( kpn I),

[0026] R1: 5'- TCTAGA ATCCAATTCCAATCCTTTGCAACG-3'( Xba I);

[0027] Amplified by PCR OsNPF5.16 After cDNA of the gene, passed kpn I. Xba After I digestion, it was connected into the pCAMBIA-1306 vector (the pCAMBIA-1306 vector was purchased from Cambia Company), and the OsNPF5.16 gene overexpression vector OsNPF5.16 -p1306. Agrobacterium EHA105 Mediated genetic transformation method, the overexpression vector was introduced into the normal rice variety Zhonghua 11.

[0028] Transplant all the obtained transgenic seedlings into baskets with soil, water and fertilize them regularly, and plant them in the field when the seedlings grow about 10cm in height. After the seedlings grow up, extract genomic DNA and detect the transge...

Embodiment 2

[0035] Example 2 OsNPF5.16 Construction of Gene Interference Plants

[0036] Extract the RNA of rice Zhonghua 11 and reverse transcribe it into cDNA, using the primer pair:

[0037] F4: 5'- GGTACC GGAGATCTCGGAGCGGTTCGCCTT-3'( Kpn I),

[0038] R4: 5'- GGATCC CATGCCAAGCCCCAGGATGT-3'( Bam H I);

[0039] F5: 5'- ACTAGT GGAGATCTCGGAGCGGTTCGCCTT-3'( Speech I),

[0040] R5: 5'- GAGCTC CATGCCAAGCCCCAGGATGT-3'( Sac I);

[0041] PCR amplified OsNPF5.16 After the cDNA fragment of the gene was digested with the corresponding restriction endonuclease, it was connected into the pTCK303 vector to construct the OsNPF5.16 gene interference expression vector OsNPF5.16 - pTCK303. Agrobacterium EHA105 Mediated genetic transformation method, introducing the interference expression vector into the normal japonica rice variety Zhonghua 11.

[0042] Transplant all the obtained transgenic seedlings into baskets with soil, water and fertilize them regularly, and plant them in...

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Abstract

The invention discloses an application of an OsNPF5.16 gene for increasing single-plant yield of paddy rice and belongs to the field of plant gene engineering. The amino acid sequence of encoding proteins of the OsNPF5.16 gene is represented as the SEQ ID No.1; and the sequence of a cDNA is represented as the SEQ ID No.2. In the method, an OsNPF5.16 gene over-expression plant and an OsNPF5.16 gene interference plant of paddy rice are constructed, and it is found that tiller number, spike number and grain filling number of normal paddy rice are increased by increasing expression of the OsNPF5.16 gene, so that the OsNPF5.16 gene can be applied to paddy rice selection breeding to increase yield of the paddy rice. The OsNPF5.16 gene has important application value of expounding influence of nitrogen on growth and development of plants and improvement on plant type of the paddy rice.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to OsNPF5.16 Application of genes in improving rice yield per plant. Background technique [0002] Plants obtain nitrogen by absorbing ammonia, nitrate, amino acids, soluble peptides, etc. in the soil; nitrogen absorption and transport mainly rely on ammonium transport protein (AMT), nitrate transport protein (NRT), amino acid transport protein (AAT) , Peptide Transporter (PTR) and other transport proteins (Williams L, Miller A. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annual Review of Plant Physiology and Plant Molecular Biology, 2001, 52: 659-688.). Ammonium is absorbed by plant AMT to synthesize glutamine and glutamate by glutamine synthase (GS) and glutamate synthase (GOGAT), which in turn form other amino acids (Sonoda Y, Ikeda A, Saiki S , et al. Feedback regulation of the ammonium transporter gene family AMT1 by glu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00C07K14/415
CPCC07K14/415C12N15/8261
Inventor 方中明钱焌杰汪杰吕凯黄玮婷
Owner WUHAN INST OF BIOENG
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