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Application of kindlin-2 protein as a target in the preparation of drugs for treating diabetes

A diabetes and protein technology, applied in the field of medicine, can solve the problem of lack of diabetes medicine and treatment methods

Active Publication Date: 2020-04-28
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is still a lack of effective drugs and treatments for diabetes in clinical practice.

Method used

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  • Application of kindlin-2 protein as a target in the preparation of drugs for treating diabetes
  • Application of kindlin-2 protein as a target in the preparation of drugs for treating diabetes
  • Application of kindlin-2 protein as a target in the preparation of drugs for treating diabetes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Immunofluorescence detection of the expression region and expression level of Kindlin-2 protein

[0063] 1. Immunofluorescence staining

[0064] Paraffin-embedded sections of two-month-old mouse pancreas were stained with insulin antibody, Kindlin-2 antibody and glucagon antibody, and observed under a confocal laser microscope. The result is as Figure 1 ~ Figure 2 shown, where figure 1 Figure (a) shows the results of insulin antibody staining, (b) shows the results of Kindlin-2 antibody staining, and (c) shows the results of co-staining of insulin antibodies and Kindlin-2 antibodies. figure 2 (a) shows the results of glucagon antibody staining, (b) shows the results of Kindlin-2 antibody staining, (c) shows the co-staining results of glucagon and Kindlin-2 antibodies, (d) Figure (a) shows the partial enlarged view of the framed area, (e) shows the staining results of DAPI (4',6-diamidino-2-phenylindole), (f) shows the square of (c) Partial zoom-in of the framed ar...

Embodiment 2

[0070] Effect of Knockout of Kindlin-2 Gene on Body Weight and Survival Rate of Mice

[0071] Kindlin-2 in mice is specifically knocked out by gene knockout technology, wherein, Kindlin-2 RIP Denotes mice that specifically knock out Kindlin-2 in islet β cells, and RIP-Cre denotes mice that are normal controls. After the knockout, the total RNA of the corresponding tissue was extracted, and then quantitatively analyzed by real-time qPCR, and the ratio of the measured total RNA to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the corresponding tissue was plotted as the ordinate, and the results were as follows Figure 6 , the results show that Kindlin-2 RIP The knockout of Kindlin-2 in mice is specific and limited to pancreatic islets, and other tissues such as liver, kidney, heart, lung, etc. have no significant difference from the normal group.

[0072] Both the experimental group and the control group began to measure the body weight of the mice one week after bir...

Embodiment 3

[0077] Effects of Knockout Kindlin-2 Gene on Fasting Blood Glucose Level and Blood Glucose Tolerance in Mice

[0078] Determination of fasting blood glucose: Two groups of mice aged two months were starved overnight, then blood was collected from the tail, and the fasting blood glucose concentration was measured with blood glucose test strips, with 5 mice in each group. The result is as Figure 10 , Kindlin-2 RIP Represents mice with specific knockout of Kindlin-2 in islet β cells, RIP-Cre represents normal control mice, the results show that Kindlin-2 RIP Fasting blood glucose levels in the mice were significantly higher than in the control group.

[0079] Glucose tolerance test (GTT): Two groups of mice aged two months were starved overnight, then intraperitoneally injected with glucose at a dose of 2 g / kg body weight, and then blood was collected at different time points to measure blood sugar, with 10 mice in each group. The result is as Figure 11, Kindlin-2 RIP Repr...

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Abstract

The invention relates to application of Kindlin-2 protein as a target spot to the preparation of medicine for treating diabetes. Research results show that expressed regions of Kindlin-2 and insulin basically coincide with each other, and the expression quantity of Kindlin-2 has closed dependency with diabetes. The mouse fasting insulin level is obviously lower than a control group after Kindlin-2 gene specificity is knocked out, glucose tolerance capacity is obviously reduced, but the sensibility to insulin of peripheral tissue after the is Kindlin-2 gene specificity is knocked out is not obviously different from that to insulin of peripheral tissue when the Kindlin-2 gene specificity is not knocked out, so that Kindlin-2 can serve as a new target spot of medicine for treating diabetes.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of a Kindlin-2 protein as a target in the preparation of medicines for treating diabetes. Background technique [0002] Diabetes mellitus is a metabolic disease characterized by hyperglycemia caused by defective insulin secretion or impaired biological action. With the improvement of people's living standards, the number of cases and prevalence of diabetes are increasing year by year. According to the latest seventh edition of the "IDF Diabetes Atlas" report (2015), there are currently about 415 million people suffering from diabetes in the world, and in China, the number of diabetic patients is about 109.6 million, accounting for 1 / 4 of the total number of diabetes in the world. It is estimated that by 2040, the number of people with diabetes in the world may reach 642 million. Whether in developed or developing countries, the situation of diabetes is very serious. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K47/69A61K47/54A61P3/10G01N33/68G01N33/50
CPCA61K45/00G01N33/5088G01N33/6893
Inventor 肖国芝曹惠玲
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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