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Detection method for early-stage hepatocellular injury diagnosis

A technology of liver cell damage and detection method, which is applied in the field of detection of early liver cell damage diagnosis, can solve the problems of liver parenchyma damage and aggravation of chronicity, and achieves the effects of good stability and high sensitivity

Inactive Publication Date: 2017-09-01
江西乐成生物医疗有限公司
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  • Abstract
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Problems solved by technology

However, when AST continues to increase and the value exceeds ALT, it often indicates severe liver parenchymal damage, which is a sign of aggravated chronicity

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  • Detection method for early-stage hepatocellular injury diagnosis

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Embodiment Construction

[0027] The embodiments of the present invention will be described in detail below, however, the embodiments of the present invention are not limited thereto. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0028] A detection method for early diagnosis of liver cell injury, comprising the following steps:

[0029] Step 1: Sample preparation, including fresh non-hemolyzed serum, anticoagulant-containing plasma, and red blood cell lysate; the red blood cell lysate sample collection method first collects blood with an anticoagulant tube, mixes it upside down, and then takes 0.5 centrifuge at 2000rpm for 5min, discard the supernatant (to remove plasma); suspend and wash the precipitate with 0.9% sodium chloride, centrifuge at 2000rpm for 5min, discard the supernatant (remove 0.9% sodium chloride), repeat the suspension ...

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Abstract

The invention discloses a detection method for early-stage hepatocellular injury diagnosis. The detection method comprises the steps of preparing a sample, preparing a kit, determining the sample, and calculating a GR energy value in the sample, wherein reduced nicotinamide adenine dinucleotide phosphate (NADPH) is adopted for donating hydrogen; GR can catalyze oxidized form glutathione (GSSG) for reducing to generate reduced glutathione (GSH); the NADPH has intensive light absorption at a 340nm wavelength part; the NADPH is oxidized to NADP<+> during reaction, the light absorption degree at the 340nm wavelength part is reduced, and the reduction rate is in direct proportion to the activity of the GR in the sample, so that an activity level of the GR can be calculated through determining the reduction rate of A340; the detection method is a simple and practicable method for detecting the GR activity in serum, plasma or other samples through an ultraviolet enzyme method, and is high in sensitivity and good in stability.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a detection method for early liver cell injury diagnosis. Background technique [0002] The liver is one of the most important organs for maintaining life. It is the largest internal organ in the human body. Its main functions involve material and energy metabolism, biological detoxification, biosynthesis, bile secretion, endocrine and immunity. Liver injury caused by any reason will affect these functions of the liver to varying degrees, and at the same time cause dysfunction of other tissues and organs. [0003] In the current clinical application, the application of liver biochemical testing items and automatic biochemical instruments is becoming more and more common. Among them, liver cell damage and the degree of damage are often judged according to SALT (serum alanine aminotransferase) and SAST (serum aspartate aminotransferase). ALT exists in the hepatic cytopl...

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Application Information

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IPC IPC(8): G01N1/38G01N21/31
CPCG01N1/38G01N21/3103
Inventor 周惠良胡海芹万蒙
Owner 江西乐成生物医疗有限公司
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