Recombinant plasmid and application thereof

Abstract

The invention provides recombinant plasmid which is constructed by introducing 2 nucleotide sequences shown as SEQ ID NO:1 into a multiple cloning site area of pGEX-6P-1 plasmid. The invention further provides application of the recombinant plasmid and a detection kit for detecting double-stranded DNA antibody. The recombinant plasmid can serve as an antigen for detecting level of dsDNA antibody so as to screen risk of to-be-detected people populations in being affected with systemic lupus erythematosus; if expression level of the dsDNA antibody is high, the risk in being affected with systemic lupus erythematosus is high; if the expression level of the dsDNA antibody is low, the risk in being affected with systemic lupus erythematosus is low. The recombinant plasmid can be used for auxiliary diagnosis of clinical systemic lupus erythematosus and has good application prospect.

Description

A recombinant plasmid and its use Technical field The invention belongs to the field of immunodiagnosis, and specifically relates to a recombinant plasmid and its use. Background technique Double-stranded DNA (dsDNA) antibodies are highly specific antibodies for systemic lupus erythematosus (SLE). Changes in antibody levels are related to disease activity. They are called "labeled antibodies" for SLE. dsDNA antibodies have important guidance for the diagnosis and prognosis of SLE. significance. The current laboratory methods for detecting dsDNA antibodies mainly include radioimmunoassay, indirect immunofluorescence, dot immunogold percolation method, immunoblotting method and enzyme-linked immunosorbent (Elisa) method. Among them, the Elisa method for detecting dsDNA antibody has the advantages of no radioisotope contamination, convenient operation, high sensitivity, and suitable for high-throughput sample determination. It is widely used in clinical practice and has good mar...

AI Technical Summary

Problems solved by technology

In the preparation of dsDNA antibody detection Elisa kit, the main technical bottleneck is the dsDNA antigen. If the dsDNA antigen is not properly selected, it will not be possible to prepare a highly specific and well-binding antigen-coated plate, and the accuracy of the Elisa kit cannot be guaranteed.

At present, almost all the Elisa kits used in the market for detecting dsDNA antibodies are imported original kits and subpackaged kits, which are expensive and costly to detect